The detrimental effects caused by the presence of biogenic amines in food samples render them an important parameter for evaluating food quality. In the current report, a facile fluorescent Biginelli based receptor 1 has been prepared through a multicomponent reaction between pyridine-2-carbaldehyde, methyl acetoacetate and 2-aminobenzimidazole in a single pot using a zinc perchlorate catalyst. Subsequently, the receptor 1 was processed to obtain aqueous phase organic nanoparticles, N1, which were characterized using DLS and TEM. Fluorescence spectroscopy studies in aqueous media revealed that the organic nanoparticles show interesting potential for Ag(I) recognition with a fluorescence “turn-off” mechanism. The resultant Ag–receptor complex, N1.Ag(I), was further found to bind with tyramine (limit of detection: 3.91 nM) and 1,2-diaminopropane (limit of detection: 4.21 nM) through unquenching of fluorescence.