Conjugation of RNA via 2′-OH acylation: Mechanisms determining nucleotide reactivity†,10.1039/D2CC00660J

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Conjugation of RNA via 2′-OH acylation: Mechanisms determining nucleotide reactivity†

Biswarup Jash,Eric T. Kool

Chemical Communications Pub Date : 02/22/2022 00:00:00 , DOI:10.1039/D2CC00660J

Abstract

The acylation reactivity of RNA 2′-OH groups has proven broadly useful for labeling and mapping RNA. Here we perform kinetics studies to test the mechanisms governing this reaction, and we find strong steric and inductive effects modulating reactivity. The results shed light on new strategies for improved conjugation and mapping.