Due to the wide usage of H₂O₂ in daily products and its adverse effects on organisms, developing effective, simple and sensitive approaches for detecting H₂O₂ in multiple real cases has always been of concern. In this paper, a pyridinic nitrogen doped carbon loaded nano-copper (N-C@Cu) sheet composite has been successfully prepared and used as an effective electrochemical material for detecting H₂O₂. The characterization of the fabricated electrode material sensor was performed by transmission electron microscopy (TEM), X-ray spectroscopy (XRD), energy-dispersive spectroscopy (EDS), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). The prepared electrode presented high sensitivity, a wide linear range, excellent repeatability and multi-day detection stability as a result of high content of pyridinic nitrogen in the N-C@Cu/Nafion/GCE. Under the optimized experimental parameters, the detection range was from 4 to 1400 μM and the limit of detection was 2.98 μM (S/N = 3). The N-C@Cu sensor has also been successfully used for detecting H₂O₂ in environmental water samples and domestic products.