Reactive adsorption desulfurization (RADS) has high desulfurization activity and low olefin saturation advantages for upgrading the fluidized catalytic cracking of gasoline. The effect of reaction temperature on RADS over the NiO/ZnO–Al₂O₃–SiO₂ adsorbent was investigated. At 350 °C, the adsorbent has good desulfurization performance but the olefin hydrogenation saturation is high, especially for C4–C7 olefins. The comparative desulfurization experiment results demonstrate that there is competitive adsorption between sulfides and olefins on the active sites. However, the amount of sulfides adsorbed by the active sites of the adsorbent is obviously more than that of olefins, which means that the hydrogenation of olefins can hardly inhibit the desulfurization reaction. The increase in Ni loading can promote the desulfurization performance but also greatly aggravate olefin hydrogenation.