Glucose detection is of great significance in the fields of clinical analysis and the food industry. Herein, a visual and portable method was developed for detecting glucose via hydrogen peroxide (H₂O₂) test strips. Once glucose oxidization was catalyzed by glucose oxidase (GOD) to produce H₂O₂, the color of the H₂O₂ test strip changed obviously. There is a good linearity for the glucose response in the range of 0.01–0.5 mM, and the limit of detection (LOD) reached as low as 0.02 mM by the naked eye. The GOD-functionalized H₂O₂ test strip was used to detect glucose in one step within 2 min. The specific catalytic oxidation of glucose by GOD ensured the high specificity for detecting glucose. The cost was calculated as within $ 0.62 per measurement. Therefore, it provides a facile, rapid and low-cost visual bioassay for glucose point-of-care testing (POCT).