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Application of analytical techniques to monitoring of kidney viability before transplantation during storage in ViaSpan® and Biolasol® Plus preservation solutions
Jerzy Skuciński,Monika Dąbrowska,Małgorzata Starek,Mirosław Szura,Jarosław Wieczorek,Zdzisław Smorąg
Analytical Methods Pub Date : 09/18/2014 00:00:00 , DOI:10.1039/C4AY01731E
Abstract

In a porcine kidney transplant model, the effects of ViaSpan® and Biolasol® Plus solutions, as cellular preservation solutions, on graft function were compared. The retrieved porcine kidneys were flushed with either ViaSpan® or Biolasol® Plus solution and cold-stored for 72 h. In the analyzed tissue samples, the ADP/ATP ratio, Caspase-3 activity and DNA fragmentation were evaluated. The changes in the individual morphological finding were observed in pig kidneys preserved in both fluids. All the obtained data suggest that for short-term cold storage of kidney grafts (0 to 24 hours) Biolasol® Plus and ViaSpan® are equally effective, and indicate that porcine kidneys were best preserved in Biolasol® Plus solution for long-term storage (48 to 72 hours). Furthermore, there was no significant difference in the modes of cell death after storage in both of the preservation solutions.

Graphical abstract: Application of analytical techniques to monitoring of kidney viability before transplantation during storage in ViaSpan® and Biolasol® Plus preservation solutions
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