This study focused on synthesizing ginger-stabilized silver nanoparticles (Gin-AgNPs) using a more eco-friendly method that utilized AgNO₃ and natural ginger solution. These nanoparticles underwent a color change from yellow to colorless when exposed to Hg²⁺, enabling the detection of Hg²⁺ in tap water. The colorimetric sensor had good sensitivity, with a limit of detection (LOD) of 1.46 μM and a limit of quantitation (LOQ) of 3.04 μM. Importantly, the sensor operated accurately without being affected by various other metal ions. To enhance its performance, a machine learning approach was employed and achieved accuracy ranging from 0% to 14.66% when trained with images of Gin-AgNP solutions containing different Hg²⁺ concentrations. Furthermore, the Gin-AgNPs and Gin-AgNPs hydrogels exhibited antibacterial effects against both Gram-negative and Gram-positive bacteria, indicating potential future applications in the detection of Hg²⁺ and in wound healing.