Iron is the main substance for maintaining life. Real-time determination of ferric ion (Fe³⁺) in living cells is of great significance for understanding the relationship of Fe³⁺ concentration changes with various physiological and pathological processes. Fluorescent probes are suitable for the detection of trace metal ions in cells due to their low toxicity and high sensitivity. In this work, a boron-dipyrromethene-based fluorescent probe (BODIPY-CL) for selective detection of Fe³⁺ was synthesized. The fluorescence emission of BODIPY-CL was determined at 516 nm. In a pH range of 1 to 10, the probe BODIPY-CL exhibits a quenching response to Fe³⁺. Meanwhile, BODIPY-CL showed a highly selective response to Fe³⁺ compared with 16 kinds of metal ions. The stoichiometry ratio of BODIPY-CL bound to Fe³⁺ was nearly 2 : 1. The fluorescence quenching response obtained by the sensor was linear with the Fe³⁺ concentration in the range of 0–400 μM, and the detection limit was 2.9 μM. BODIPY-CL was successfully applied to image Fe³⁺ in cells. This study provides a promising fluorescent imaging probe for further research on the physiological and pathological effects of Fe³⁺.