A C-di-GMP–proflavine–hemin supramolecular complex has peroxidase activity—implication for a simple colorimetric detection†,10.1039/C2MB05430B

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A C-di-GMP–proflavine–hemin supramolecular complex has peroxidase activity—implication for a simple colorimetric detection†

Shizuka Nakayama,Kevin Roelofs,Vincent T. Lee,Herman O. Sintim

Molecular BioSystems Pub Date : 01/18/2012 00:00:00 , DOI:10.1039/C2MB05430B

Abstract

Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP–proflavine–hemin nucleotidezyme can oxidize the colorless compound 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS˙⁺ and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.