Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity†‡
Wenjuan Zha,Sheryl B. Rubin-Pitel,Huimin Zhao
Molecular BioSystems Pub Date : 01/31/2008 00:00:00 , DOI:10.1039/B717705D
Abstract
Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescensPf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.
