Synthesis and growth-inhibitory activities of imidazo[5,1-d]-1,2,3,5-tetrazine-8-carboxamides related to the anti-tumour drug temozolomide, with appended silicon, benzyl and heteromethyl groups at the 3-position†‡,10.1039/C7MD00554G

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Synthesis and growth-inhibitory activities of imidazo[5,1-d]-1,2,3,5-tetrazine-8-carboxamides related to the anti-tumour drug temozolomide, with appended silicon, benzyl and heteromethyl groups at the 3-position†‡

David Cousin,Marc G. Hummersone,Tracey D. Bradshaw,Jihong Zhang,Christopher J. Moody,Magdalena B. Foreiter,Helen S. Summers,William Lewis,Richard T. Wheelhouse,Malcolm F. G. Stevens

MedChemComm Pub Date : 01/19/2018 00:00:00 , DOI:10.1039/C7MD00554G

Abstract

A series of 3-(benzyl-substituted)-imidazo[5,1-d]-1,2,3,5-tetrazines (13) and related derivatives with 3-heteromethyl groups has been synthesised and screened for growth-inhibitory activity in vitro against two pairs of glioma cell lines with temozolomide-sensitive and -resistant phenotypes dependent on the absence/presence of the DNA repair protein O⁶-methylguanine-DNA methyltransferase (MGMT). In general the compounds had low inhibitory activity with GI₅₀ values >50 μM against both sets of cell lines. Two silicon-containing derivatives, the TMS-methylimidazotetrazine (9) and the SEM-analogue (10), showed interesting differences: compound (9) had a profile very similar to that of temozolomide with the MGMT+ cell lines being 5 to 10-fold more resistant than MGMT− isogenic partners; the SEM-substituted compound (10) showed potency across all cell lines irrespective of their MGMT status.