ABSTRACTStable isotope ratios of nitrogen and oxygen (15N/14N and 18O/16O) of nitrate (NO3–) are excellent tracers for developing systematic understanding of sources, conversions, and deposition of reactive atmospheric nitrogen (Nr) in the environment. Despite recent analytical advances, standardized sampling of NO3–) isotopes in precipitation is still lacking. To advance atmospheric studies on Nr species, we propose best-practice guidelines for accurate and precise sampling and analysis of NO3– isotopes in precipitation based on the experience obtained from an international research project coordinated by the International Atomic Energy Agency (IAEA). The precipitation sampling and preservation strategies yielded a good agreement between the NO3– concentrations measured at the laboratories of 16 countries and at the IAEA. Compared to conventional methods (e.g., bacterial denitrification), we confirmed the accurate performance of the lower cost Ti(III) reduction method for isotope analyses (15N and 18O) of NO3– in precipitation samples. These isotopic data depict different origins and oxidation pathways of inorganic nitrogen. This work emphasized the capability of NO3– isotopes to assess the origin and atmospheric oxidation of Nr and outlined a pathway to improve laboratory capability and expertise at a global scale. The incorporation of other isotopes like 17O in Nr is recommended in future studies.

(2022). Dr Ingeborg Maaß. Isotopes in Environmental and Health Studies: Vol. 58, No. 1, pp. 111-112.

ABSTRACTThe stable isotope ratios of carbon and nitrogen (δ13C and δ15N) were measured in composite samples of Japanese food and hair. Three hundred eighty-nine foodstuffs were collected in Tokyo and Gunma Prefecture, Japan, in 2020. The foodstuffs were classified into 15 food categories, prepared as usually consumed, and mixed to make 15 composite samples representing each of the food categories. Similarly prepared samples for foodstuffs collected in 2011 and 2015 were also examined. Composite hair samples were collected from a barber shop in Tokyo and a beauty salon in Gunma in 2019. The δ13C and δ15N values of the food and hair composites were measured by elemental analyzer/isotope ratio mass spectrometry after defatting. The δ13C and δ15N values of the food composite varied from composite to composite and according to year of collection. The whole-diet δ13C values were −21.1, −22.0, and −21.5 ‰ for the 2011, 2015, and 2020 samples, respectively; the δ15N values were 5.0, 4.4, and 4.4 ‰, respectively. Diet–hair offset values of δ13C and δ15N were calculated to be 1.9 and 4.3 ‰ for δ13C and δ15N, respectively. These offset values will be important for dietary analysis and nutritional research using hair isotope ratios.

ABSTRACTGas samples were collected from 25 m long horizontal boreholes drilled into the excavation field at 10° inclination to the longwall face in two mining areas, Pesje and Preloge, in the Velenje Coal Mine, Slovenia, from 2013 to 2018. The degassing mechanism of coalbed gas and its stable isotopic composition (δ13CCO2, δ13CCH4, and δ2HCH4) were investigated in boreholes in advance of eight working faces. The major coalbed gas constituents were CO2 and methane. Gas concentrations and isotope values revealed that the methane is biogenic in origin with δ13CCH4 values of −69.4 to −29.5 ‰, δ2HCH4 values of −301 to −222 ‰, and a fractionation factor (αCO2-CH4) of 0.998–1.073, suggesting that methane derives from microbial acetate fermentation and CO2 reduction. The carbon dioxide methane index values ranged from 50.0–98.3 vol.% and δ13CCO2 values from −11.8 to −0.5 ‰, indicating that CO2 is biogenic and endogenic in origin. The degassing mechanism results in isotope fractionation of methane and CO2 for carbon isotopes up to 39.9 ‰ and up to 8.5 ‰, respectively, depending on the position of the excavation fields in space, e.g. under pre-mined coal area, fresh overburden.

ABSTRACTThe objective of this work is to propose a more effective way to prepare an in-house CO2 with known triple oxygen isotope compositions. The major experimental steps include: (1) the O2 is combusted to CO2 on a graphite rod at 750 °C with Pt-catalyst for 3–4 min; and (2) converted CO2 is subsequently purified by two cryogenic traps. The results show high reproducibility of δ13C and δ18O values of the converted CO2 within 0.010–0.020 ‰ and 0.006–0.010 ‰ (1σ, SD), and the identical δ18O value within error with that of the original O2. Additionally, we have measured the triple oxygen isotope compositions of converted CO2 using an O2–CO2 Pt-catalyzed oxygen-isotope equilibration method. The measured δ17O values of CO2 show high reproducibility within 0.006 ‰ (1σ, SD), and are identical within error with the original O2 as well. Notably, our experiments also found that the O2 with heavier oxygen isotope ratios (δ18O > 40 ‰, VSMOW) might have a lesser conversion efficiency, and this effect, combined with the lighter isotope preferential fractionations during the reaction processes of O2 to CO and CO to CO2, may explain the observed lower 17O/16O and 18O/16O ratios of the converted CO2 relative to the original O2.

ABSTRACTThe Upper Cibolo Creek (UCC) karst watershed in Central Texas, USA, represents a portion of the drainage area that supplies water to the recharge zone for the Edwards Aquifer. However, the surface water–groundwater interactions along the UCC are not well quantified, and the hydraulic interactions are important for water budget and water quality of the aquifer. In this study, we investigated the evolution of hydrochemical and isotopic signatures (δ18O, δ2H and d-excess) from precipitation, surface water to groundwater in the UCC watershed from 2017 to 2019, and investigated surface water–groundwater interactions using samples from 14 creeks/spring sites. Factor analysis for the observed parameters demonstrates that changes in water hydrochemistry are primarily controlled by human activity, precipitation input, and water–rock interaction. Hierarchical clustering analysis of temporal isotope variations confirms that significant surface water–groundwater interactions occur in the UCC watershed. We identified relationships between nitrate concentrations at creek/spring sites and land-use conditions, and nitrate input sources were determined utilizing the dual-isotope analyses (δ15N and δ18O) of nitrate. This study provides capacity for a more precise assessment of water resources and water quality in Central Texas.

ABSTRACTLake Sevan is a meso-eutrophic water body, which was severely impacted by anthropogenic level decrease, pollution and eutrophication during the last century. Starting in the 1970s, these processes resulted in the formation of an oxygen-depleted hypolimnion during summer–autumn stratification of the lake. In this work, we demonstrate for the first time that eutrophication of the lake leads not only to the full depletion of oxygen and nitrate in the hypolimnion but as well to the presence of sulfate-reducing microorganisms and toxic hydrogen sulfide. Concentrations of hydrogen sulfide in the hypolimnion of Major and Minor Sevan in October were as high as 9 and 39 μM, respectively. In October 2019, 66 % of lake's bottom was covered by sulfidic waters, while the fraction of sulfidic water volume reached 19 %. Values of δ34S for hypolimnetic sulfide are lower by only 7–12 ‰ compared to epilimnetic sulfate, while δ33S values of sulfide are similar to the δ33S values of sulfate. These isotopic fingerprints are not consistent with microbial sulfate reduction as the sole source of hydrogen sulfide in the hypolimnion. We attribute the formation of a sulfidic deep-water layer to a combination of microbial sulfate reduction in the water column and diffusion of hydrogen sulfide from the sediments.

ABSTRACTThe authors studied the atmospheric radon concentration with associated meteorological parameters variation during the dust events from July to November 2017. We obtained the meteorological parameters data in weather station of Sulaymaniyah city, Iraq. In the environmental monitoring plan, the atmospheric radon fluctuated from 15 to 48 Bq m–3 around the mean value of 31.5 ± 7 Bq m–3 within the summer. In autumn, varied from 22 to 46 Bq m–3 with a mean value of 34 ± 12 Bq m–3. We employed this to determine the radon level anomalously. Using the modified statistical coefficients, such as the residual deviation (RD), residual fluctuation ratio (RFR), F-test, and p-value coefficients. Among the atmospheric radon fluctuation values, particularly one anomalous (42 Bq m–3) on 25 July was determined because the excessive value of the RD was 1.9 σ, and the RFR value was 66 %. Corresponding to our coefficients criteria, the minimum level of atmospheric radon (22 Bq m–3) does not consider anomalous because of increasing wind speed. Based on this, our method for determining the atmospheric radon anomalies that are influenced by the missed factors beyond the mentioned meteorological parameters is accurate.

ABSTRACTFor the first time, a map of the 222Rn gas has been produced in the Campania region, the southern part of Italy, based on the activity concentration measured in indoor environments. This work is part of the radon mitigation policy and complies with the recent Italian Legislative Decree 101/2020, which implements the European Basic Safety Standards, Euratom Directive 59/2013, where Member States must declare areas with elevated levels of indoor radon concentration. The obtained map, divided by Campania municipalities, identifies priority areas with activity concentration values exceeding the reference level of 300 Bq m–3. In addition, an effective statistical analysis of the dataset has been carried out.

ABSTRACTShallow groundwater and lake water are the dominant water resources in the Badain Jaran Desert. There are still controversial hypotheses related to the origin of groundwater in this desert. Few studies have been conducted to explore the Sr provenance of these waters and assess the water–rock interactions using a Sr isotope approach until now. In this text, the Sr isotope data of waters in the hinterland of the Badain Jaran Desert and neighbouring areas are reported. The waters in the Badain Jaran Desert have few links to its surrounding rivers, but could be influenced by the precipitation in the Yabulai Mountains. The 87Sr/86Sr ratio changes constantly, while the Sr2+ concentration of shallow groundwater gradually decreases from Yabulai to the desert hinterland to Gurinai-Guaizihu. Combined with hydrochemical data and hydrodynamic conditions, these results show that the dissolved Sr of waters in the desert hinterland is controlled by the Yabulai precipitation and catchment weathering. They further show that the desert shallow groundwater Sr originates mainly from the Yabulai precipitation (> 94 %), while whole-rock weathering contributes little (< 6 %), as calculated using isotope mass balance equations. Relative Sr contributions to lakes from shallow groundwater and catchment weathering are calculated to be 92.5 and 7.5 %, respectively.

ABSTRACTAlthough stable hydrogen (δ2H) and carbon (δ13C) isotopic compositions of bird feathers have been increasingly used to understand the migration of birds through assignment of individuals to established isoscapes, far less is known about the structure of feather isoscapes based on stable sulphur isotope (δ34S) assays. While δ2H isoscapes have been used to investigate the migratory origins of the Globe Skimmer (Pantala flavescens) dragonfly in India, no isotope studies have been applied to avian migration patterns in that region. Here, based on museum collections, we report the feather δ34S values of 13 avian species that included marine birds with expected 34S enriched feathers, resident birds throughout India and a single migratory species, Bar-headed Goose (Anser indicus). Feathers from the marine birds were the most enriched (20.6 ± 1.2 ‰, n = 10; range = 4.4 ‰) in 34S but terrestrial resident species also showed unexpectedly high δ34S values (11.7 ± 4.8 ‰, n = 74; range = 19.9 ‰) throughout most of India but the Himalayan region and Gangetic plain typically showed δ34S values less than 6.4 ‰. Our results show the utility of feather δ34S studies to decipher avian and other animal migration between these two regions.

ABSTRACTSoil gas radon and indoor radon measurements have been carried out in Mayo-Louti and Benoué Divisions in northern Cameroon. Concentrations of radon in soil have been measured using Markus 10 at the depth of about 1 m. Radon concentration in soil varies from 0.9 to 13.8 kBq m−3 with a mean value of 4.6 kBq m−3. Average daily indoor radon concentrations measured with RadonEye+2 detectors vary from 7 to 60 Bq m−3 with an average of 17 Bq m−3. Indoor radon concentrations measured with passive RADTRAK detectors range between 15 and 104 Bq m−3 with a geometric value of 38 Bq m−3 and a geometric standard deviation of 1.5. This geometric value is lower than the value of 30 Bq m–3 given by UNSCEAR. Indoor radon inhalation dose ranges between 0.28 and 1.97 mSv a−1 with geometric value of 0.72 mSv a−1 (at 0.03 standard deviation). Outdoor radon inhalation ranges between 0.02 and 0.26 mSv a−1 with a mean value of 0.09 mSv a−1. The total annual effective dose due to indoor and outdoor radon exposure for this study area is 0.81 mSv a−1, less than 1.15 mSv a−1 the world average value given by UNSCEAR. There is no significant radiological risk for the inhabitants.

ABSTRACTNitrate pollution has become an environmental problem of global concern. One effective way for controlling the nitrate pollution of water is to identify the pollution source and reduce the input of nitrate. This study traces and quantifies the sources of nitrate contamination to groundwater and surface water in the northeastern suburbs of Beijing, where an emergency groundwater source zone is located. Nitrogen and oxygen stable isotope analysis, geospatial analysis techniques, principal component analysis, correlation analysis, and a Bayesian isotope mixing model were used to achieve our goals. The results show that the main sources of nitrate pollution in groundwater were manure and sewage (M&S) (42.6 %) > soil nitrogen (SN) (26.6 %) > NH4+ in fertilizer and rain (NHF&R) (24.5 %) > NO3− fertilizer (NOF) (5.0 %) > NO3− in atmospheric deposition (NAD) (1.3 %), and main sources of nitrate in surface water were M&S (28.8 %) > SN (20.4 %) > NAD (19.8%) > NOF (16.5%) > NHF&R (14.5 %). Due to the high permeability of the aquifer in the study area, there was a strong hydraulic connection between groundwater and surface water. The discharge of treated wastewater (reclaimed water) into the mostly dried river channel in the study area might aggravate nitrate pollution in the groundwater.

ABSTRACTThis study determined the main local and regional parameters affecting the stable isotopes (18O, 2H) in the Bangkok precipitation and developed the Bangkok meteoric water line (BMWL) (δ2H = (7.68 ± 0.07) δ18O + (7.25 ± 0.48)). First, Pearson correlation coefficients were used to determine the correlation between local and regional parameters. Six different regression methods were used based on Pearson correlation coefficients. The stepwise regression had the most accurate performance among them according to the R2 values. Second, three different methods were used to develop the BMWL, and their performances were also studied. Third, the stepwise regression method was used to study the effects of local and regional parameters on the stable isotope content of precipitation. The results showed that the local parameters had a greater effect on the stable isotope content than the regional ones. The stepwise models developed based on the northeast and southwest monsoons showed that moisture sources also affected the stable isotope content of precipitation. Finally, the developed stepwise models were validated by calculating the root mean square error (RMSE) and R2. This study demonstrated that the local parameters mainly controlled the stable isotopes in the Bangkok precipitation, while the regional parameters had a slight effect on them.

ABSTRACTInvestigating the sources, migration and proportional contribution of nitrate is essential to effectively protect water quality. δ15N-NO3–, δ18O-NO3– and Stable Isotope Analysis in R (SIAR) were used to qualitatively and quantitatively analyse nitrate sources in the Pingzhai Reservoir water body. The values of δ15N-NO3– and δ18O-NO3– in water vary with season. Soil organic nitrogen and chemical fertilisers are the main sources of nitrate in autumn, while domestic sewage and livestock manure are the primary sources of nitrate in winter and spring. The SIAR results showed that chemical fertilisers, livestock manure, sewage, and soil organic nitrogen had the highest proportional contribution. In autumn, the proportional contribution of chemical fertilisers to river and reservoir were 47 and 51 %. During winter, the proportional contributions of livestock manure and sewage to river and reservoir were 53 and 68 %, respectively, and in spring 49 and 68 %, respectively. Considering the fragility of karst ecosystems, strict measures should be formulated for the use of chemical fertilisers and standards for sewage discharge should be raised. Control nitrogen input from agricultural activities and prevent water quality deterioration.

ABSTRACTRainforests play an important role in hydrological and carbon cycles, both at regional and global scales. They pump large quantities of moisture from the soil to the atmosphere and are major rainfall hotspots of the world. Satellite-observed stable water isotope ratios have played an essential role in determining sources of moisture in the atmosphere. Satellites provide information about the processes involving vapour transport in different zones of the world, identifying sources of rainfall and distinguishing moisture transport in monsoonal systems. This paper focuses on major rainforests of the world (Southern Amazon, Congo and Northeast India) to understand the role of continental evapotranspiration in influencing tropospheric water vapour. We have used satellite measurements of 1H2H16O/1H216O from Atmospheric InfraRed Sounder (AIRS), evapotranspiration (ET), solar-induced fluorescence (SIF), precipitation (P), atmospheric reanalysis-derived moisture flux convergence (MFC) and wind to discern the role of ET in influencing water vapour isotopes. A global map of the correlation between δ2Hv and ET-P flux indicates that densely vegetated regions in the tropics show the highest positive correlation (r > 0.5). Using mixing models and observations of specific humidity and isotopic ratio over these forested regions, we discern the source of moisture in pre-wet and wet seasons.

ABSTRACTEurasian red squirrels Sciurus vulgaris have unusually high δ13C values compared to other forest rodents, which is seemingly related to the consumption of 13C-enriched conifer seeds. To test this hypothesis, we analyzed the hair of wild and zoo-kept red squirrels, seeds of pine and spruce, and feathers of specialized and opportunistic consumers of conifer seeds, crossbills Loxia spp., and woodpeckers Dendrocopos major. Data on the isotopic composition (δ13C and δ15N values) of hair or feathers of other species of forest rodents and birds were obtained from published studies. The range of mean δ13C values of hair of wild forest rodents (19 species) exceeded 10 ‰. All squirrel species had high 13C content, S. vulgaris having maximum δ13C values. In contrast, S. vulgaris kept in captivity had an isotopic composition similar to other captive rodents. The feathers of crossbills were enriched in 13C compared to other forest birds (15 species), while seeds of coniferous trees often had higher δ13C values compared to seeds of other woody plants. Distinctiveness of the isotopic composition of mammals and birds feeding on the seeds of coniferous trees suggests that this resource can be traced through the entire forest food web.

ABSTRACTThe isotope biogeochemistry of bioindicators has widely demonstrated its added value in environmental issues by allowing to precisely identify sources of contamination. Most of the studies are based on studying one or two isotope systematics. Here, we are presenting an innovative multi-proxy approach that combines chemistry with both stable (C, S, N) and radiogenic (Pb) isotope systematics. Using Hypogymnia physodes bioindicators, we evaluated air quality in the complex environment of the Świętokrzyski National Park (ŚNP, Poland) with the ultimate objective of isotopically identifying the sources responsible for the observed contamination. Combining the isotope systematics showed that home heating is a major source of contamination in winter, whereas the contribution of road traffic increases during the summer. Pb isotope ratios identified industrial activities as the major source of this metal in the atmosphere.

ABSTRACTLeaf nitrogen (N) status and stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) were used to study environmental factors that control mountain individuals of Picea neoveitchii trees, a coniferous species endemic and endangered in China. From May to September 2016, we carried out observations at four different altitude locations extending southeast of Daba Mountain in western Hubei Province. Needle-shaped leaf δ13C was positively correlated with needle N and C content calculated from the needle area (Narea and Carea content), needle δ15N, needle mass, and leaf mass per area (LMA), respectively. Needle δ15N was also positively correlated with monthly temperature and precipitation for the current month and last month. The seasonal normalised difference vegetation index (NDVI) was highest in June at the lowest altitude and August at the highest altitude. We found that N availability as an important driving factor for tree growth is controlled by surface soil temperature, while in summer, air temperatures above 23 °C exceed the physiological threshold of trees and limit the growth of trees. We concluded that the negative effect of higher temperature on tree growth is greater than the positive effect of higher nitrogen.

ABSTRACTIndoor radon and thoron survey has been carried out in 50 dwellings under Kohima district, Nagaland, India, using the latest measurement technology. The survey has been carried out for a one-year period in 3 different seasons, and the dwellings were selected according to the building materials used for construction. Indoor radon and thoron concentrations, as well as their progeny, followed a predictable pattern with greater levels in the winter and lower levels in the summer. Concrete housing had greater radon and thoron concentrations than bamboo and semi-wood/bamboo homes. The equilibrium factor (E.F.) and inhalation dose due to radon, thoron, and their corresponding progeny were also studied in the present study.