2. Metabolic Enzyme/Protease Cell Cycle/DNA Damage Apoptosis
  3. Herbicide DNA/RNA Synthesis Apoptosis
  4. 2,4-D ammonium

2,4-D ammonium  (Synonyms: 2,4-Dichlorophenoxyacetic acid ammonium)

目录号: HY-W547534
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2,4-D (2,4-Dichlorophenoxyacetic acid) ammonium 是一种可用于阔叶杂草防治的选择性除草剂。2,4-D ammonium 可诱导细胞凋亡 (apoptosis)。2,4-D ammonium 抑制 DNA 和蛋白质的合成，从而阻止植物的正常生长发育。

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2,4-D ammonium

2,4-D ammonium Chemical Structure

CAS No. : 2307-55-3

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2,4-D ammonium 的其他形式现货产品:

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2,4-D ammonium 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

2,4-D (2, 4-dichlorophenoxyacetic acid) ammonium is a selective herbicide that can be used for the control of broad-leaved weeds. 2,4-D ammonium can induce apoptosis. 2,4-D ammonium inhibits DNA and protein synthesis, thereby preventing normal plant growth and development[1][2][3].

体外研究
(In Vitro)

2,4-D (25-200 μM, 72 h) ammonium 抑制 A549 和 WI38 的细胞活力，IC50 值分别为 126 µM 和 115 µM。通过影响细胞周期，诱导细胞凋亡[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

2,4-D ammonium 相关抗体:

Cell Viability AssayWestern Blot AnalysisCell Proliferation AssayApoptosis AnalysisCell Cytotoxicity AssayCell Cycle AnalysisRT-PCRCell Autophagy AssayImmunofluorescenceCell Differentiation AssayCell Invasion AssayCell Migration Assay Real Time qPCRELISA Assay[2]

Cell Line: A549, W138
Concentration: 25, 50, 75, 100, 150, 200 μM
Incubation Time: 72 h
Result: Inhibited cell viability in a dose-dependent manner.

Cell Cycle Analysis[2]

Cell Line: A549
Concentration: 50, 100, 150 μM
Incubation Time: 72 h
Result: The G0/G1 population decreased to about 57%, 43% and 31%, respectively.

Cell Viability AssayWestern Blot AnalysisCell Proliferation AssayApoptosis AnalysisCell Cytotoxicity AssayCell Cycle AnalysisRT-PCRCell Autophagy AssayImmunofluorescenceCell Differentiation AssayCell Invasion AssayCell Migration Assay Real Time qPCRELISA Assay[2]

Cell Line: A549, W138
Concentration: 100, 150 μM
Incubation Time: 72 h
Result: The early apoptotic population increased to 44% in A549 cells and 57% in WI38 cells, and the late apoptotic population increased to 18% in A549 cells and 10% in WI38 cells.

Western Blot Analysis[2]

Cell Line: A549, W138
Concentration: 100, 150 μM
Incubation Time: 72 h
Result: Up-regulated p53 and Bax proteins, down-regulated Bcl-2 and procaspase 3.
体内研究
(In Vivo)

2,4-D (100 或 200 mg/kg, 灌胃, 连续30 天) ammonium 对雄性大鼠生殖系统有毒性作用[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult Wistar male rats[3]
Dosage: 100 or 200 mg/kg
Administration: i.g.
Result: Decreased the testis, seminal vesicles and prostate relative weight.
Decreased the number of spermatozoa and sperm motility.
Increased the level of FSH and LH.
分子量

238.07

Formula

C8H9Cl2NO3
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

2,4-D ammonium 相关分类

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2,4-D2307-55-32,4-Dichlorophenoxyacetic acidHerbicideDNA/RNA SynthesisApoptosisabscisic acidcaspase-3apoptosistobacco BY-2 suspension cellp53DNA ligase 4ethylene metabolic pathwaysnon-homologous end joiningzebrafish larvaeDNA repairInhibitorinhibitorinhibit

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