2. Others Neuronal Signaling
  3. Fluorescent Dye Cholinesterase (ChE)
  4. AD-BChE/HClO

AD-BChE/HClO 是一种双靶点双光子荧光探针。AD-BChE/HClO 可通过丁酰胆碱酯酶介导 7 位酯键水解，还可通过次氯酸介导硫羰基氧化，从而释放 4-甲基伞形酮。AD-BChE/HClO 可通过尾静脉注射在神经细胞和小鼠脑组织中实现双光子成像。AD-BChE/HClO 可用于阿尔茨海默病的研究。

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AD-BChE/HClO

AD-BChE/HClO Chemical Structure

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AD-BChE/HClO 相关抗体

Top Publications Citing Use of Products

查看 Cholinesterase (ChE) 亚型特异性产品:

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AChE BChE

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

AD-BChE/HClO is a dual-target two-photon fluorescent probe. AD-BChE/HClO can release 4-methylumbelliferone via butyrylcholinesterase-mediated hydrolysis of the ester bond at position 7, as well as hypochlorous acid-mediated thiocarbonyl oxidation. AD-BChE/HClO enables two-photon imaging in nerve cells and mouse brain tissues via tail vein injection. AD-BChE/HClO can be used for the research of Alzheimer's disease[1].

IC50 & Target

BChE

 

体外研究
(In Vitro)

AD-BChE/HClO (AND 逻辑门探针，Probe S1) (10 μM; up to 80 min) 是一种 AND 逻辑门探针，仅在同时存在 BChE 和 HClO 时才会产生强荧光；在无细胞生化分析中，其对 BChE 的检测限为 8.28 U/L，对 HClO 的检测限为 3.09 μM[1]。
AD-BChE/HClO (AND 逻辑门探针，探针 S1) (0-100 μM; 24 h for cytotoxicity, 40 min for imaging) 对 PC12 细胞无细胞毒性，浓度最高可达 100 μM；并且它可特异性检测外源性和内源性 HClO、上调的丁酰胆碱酯酶 (BChE)，还能通过双光子荧光成像区分 Aβ1-42 诱导或谷氨酸诱导的 AD 模型 PC12 细胞与正常细胞[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

AD-BChE/HClO 相关抗体:

Cell Cytotoxicity Assay[1]

Cell Line: PC12 cells
Concentration: 0-100 μM (cytotoxicity assay); 10 μM (imaging assays); 50-200 μM HClO; 200-500 μM hydrocortisone; 5-20 μM Aβ1-42; 5-20 mM L-glutamate
Incubation Time: 24 h (cytotoxicity assay; L-glutamate stimulation); 40 min (probe incubation for imaging); 30 min (HClO stimulation); 48 h (hydrocortisone stimulation; Aβ1-42 stimulation)
Result: Maintained PC12 cell viability above 80% after 24 h incubation at concentrations from 0 to 100 μM.
Showed increased fluorescence intensity with increasing exogenous HClO concentration.
Increased fluorescence due to LPS-induced endogenous HClO production, which was reduced by ABH treatment.
Increased fluorescence intensity due to hydrocortisone-induced BChE upregulation, which was reduced more significantly by BChE inhibitor iso-OMPA than by AChE inhibitor donepezil.
Showed 2-3 fold higher fluorescence intensity in Aβ1-42-induced and glutamate-induced AD cell models than in normal cells, which was reduced by treatment with ABH or iso-OMPA.
分子量

260.31

Formula

C14H12O3S
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

AD-BChE/HClO 相关分类

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  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AD-BChE/HClOFluorescent DyeCholinesterase (ChE)neural cellsAβ1-42glutamatehypochlorous acidbutyrylcholinesterasemouse brainsPC12 cells4-methylumbelliferonealzheimer’s diseaseInhibitorinhibitorinhibit

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