2. Apoptosis
  3. Apoptosis Caspase
  4. Anti-Human/Mouse CD95 Antibody (HFE7A)

Anti-Human/Mouse CD95 Antibody (HFE7A) 是一种靶向人/小鼠 Fas (CD95) 的抗体，对小鼠的 Kd 为 1.6 nM。Anti-Human/Mouse CD95 Antibody (HFE7A) 可调控 Fas 介导的凋亡信号通路，且不会阻断 Jo2-Fas 的结合。Anti-Human/Mouse CD95 Antibody (HFE7A) 可抑制 Jo2 诱导的 caspase 激活、线粒体去极化、肝细胞死亡及凋亡 (apoptosis)。Anti-Human/Mouse CD95 Antibody (HFE7A) 可保护 BALB/c 小鼠免受 Jo2 诱导的急性肝损伤，并降低 Jo2 相关的血清转氨酶水平升高。Anti-Human/Mouse CD95 Antibody (HFE7A) 可用于肝损伤的相关研究。同型对照可参考 Mouse IgG1 kappa, Isotype Control (HY-P99977)。

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Anti-Human/Mouse CD95 Antibody (HFE7A)

Anti-Human/Mouse CD95 Antibody (HFE7A) Chemical Structure

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Anti-Human/Mouse CD95 Antibody (HFE7A) 相关抗体

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Anti-Human/Mouse CD95 Antibody (HFE7A) is an antibody targeting human/mouse Fas (CD95), with a Kd of 1.6 nM in mice. Anti-Human/Mouse CD95 Antibody (HFE7A) modulates the Fas-mediated apoptotic signaling pathway without blocking the binding of Jo2 to Fas. Anti-Human/Mouse CD95 Antibody (HFE7A) inhibits Jo2-induced caspase activation, mitochondrial depolarization, hepatocyte death and apoptosis. Anti-Human/Mouse CD95 Antibody (HFE7A) protects BALB/c mice against Jo2-induced acute liver injury and reduces Jo2-associated elevation of serum transaminase levels. Anti-Human/Mouse CD95 Antibody (HFE7A) can be used in studies related to liver injury. For isotype control, refer to Mouse IgG1 kappa, Isotype Control (HY-P99977)[1].

同型

Mouse IgG1 kappa
推荐同型对照抗体
反应种属

Human/Mouse
体外研究
(In Vitro)

HFE7A (0.05-5 μg/mL; 1.5-6 h) 可在体外以剂量依赖的方式抑制 Jo2 诱导的原代 BALB/c 小鼠肝细胞死亡，以及 caspase-3、-8 和-9 的活化[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Human/Mouse CD95 Antibody (HFE7A) 相关抗体:

Cell Viability AssayWestern Blot AnalysisCell Proliferation AssayApoptosis AnalysisCell Cytotoxicity AssayCell Cycle AnalysisRT-PCRCell Autophagy AssayImmunofluorescenceCell Differentiation AssayCell Invasion AssayCell Migration Assay Real Time qPCRELISA Assay[1]

Cell Line: Jo2 treated hepatocytes
Concentration: 0.05, 0.5, 5 μg/mL
Incubation Time: 1.5, 3, 6 h
Result: Inhibited cell death in a dose-dependent manner.
体内研究
(In Vivo)

Anti-Human/Mouse CD95 Antibody (HFE7A) (16.5-200 μg；静脉注射；单次给药) 可完全挽救 BALB/c 小鼠免受 Jo2 诱导的致死性暴发性急性肝炎影响，并通过抑制 caspase 活化和线粒体去极化来阻断 Jo2 诱导的肝细胞凋亡[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (female)[1]
Dosage: 67 μg (survival; AST/ALT inhibition); 16.5 μg (caspase suppression); 200 μg (mitochondrial depolarization reduction)
Administration: i.v.; single dose
Result: Achieved 100% survival (3/3 mice) at 4 and 24 hours post-Jo2 injection.
Inhibited Jo2-induced increases in serum AST and ALT levels.
Almost completely suppressed Jo2-induced activation of caspases-3, -8, and -9 in hepatocytes to basal levels.
Reduced the percentage of hepatocytes with mitochondrial depolarization when administered simultaneously with Jo2 at 200 μg.
基因 ID

355  [NCBI]

Accession

P25445
靶点

Fas/CD95
应用

ELISA, FACS, Functional assay, Research in vivo
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
组分

Please refer to the lot-specific COA for specific buffer information.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Anti-Human/Mouse CD95 Antibody (HFE7A) 相关分类

  • 摩尔计算器

  • 稀释计算器

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Human/Mouse CD95 Antibody (HFE7A)ApoptosisCaspaseacute hepatic injurycaspase activationBALB/c micemitochondrial depolarizationapoptosisFas (CD95)Fas-mediated apoptotic signalingmouse thymocyteshepatocyte cell deathhuman rheumatoid arthritis synovial cellsInhibitorinhibitorinhibit

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