2. 抗体
  3. 一抗
  4. 多克隆抗体
  5. 4 Hydroxynonenal 抗体

4 Hydroxynonenal Antibody 是一个兔来源、无偶联标记、抗 4 Hydroxynonenal 的 IgG 多克隆抗体。

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规格 价格 是否有货 数量
10 μL ￥520 In-stock
50 μL ￥1350 In-stock
100 μL ￥2200 In-stock
250 μL   询价  

* Please select Quantity before adding items.

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Top Publications Citing Use of Products

    4 Hydroxynonenal Antibody purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2025 Oct 13:e06497.  [Abstract]

    4 Hydroxynonenal Antibody (diluted 1:400). Representative 4‐HNE‐stained images of ovarian tissues from the indicated groups of mice (scale bar, 20 µm).

    4 Hydroxynonenal Antibody purchased from MCE. Usage Cited in: Redox Biol. 2025 May 29:85:103672.  [Abstract]

    IHC staining of 4-HNE, and GPX4 in xenograft tumors across different treatment groups.

    4 Hydroxynonenal Antibody purchased from MCE. Usage Cited in: Mater Today Bio. 2025 Sep 18:35:102317.  [Abstract]

    4 Hydroxynonenal Antibody (1: 500). Representative immunohistochemical staining of 4-HNE and GPX4 in tumor sections from each treatment group (Ctrl, FGN, FGN-BBN, FGN+NIR, and FGN-BBN+NIR). Scale bar: 50 μm.

    4 Hydroxynonenal Antibody purchased from MCE. Usage Cited in: Cell Death Dis. 2025 Nov 7;16(1):810.  [Abstract]

    Representative images of H&E staining and IHC staining for SETD7, Ki67, ALDH1A3, and 4-HNE in subcutaneous xenograft tumors. Scale bar: 50 μm.

    4 Hydroxynonenal Antibody purchased from MCE. Usage Cited in: Free Radic Biol Med. 2024 Nov 8:226:29-42.  [Abstract]

    Representative IHC staining images showing levels of LRRC45 and 4-HNE (4 Hydroxynonenal) in bladder cancer tissues. The scale bars represent 200 μm (low magnification) and 50 μm (high magnification).

    4 Hydroxynonenal Antibody purchased from MCE. Usage Cited in: J Future Foods. 2025 Oct 27.

    Representative immunohistochemical staining of 4-HNE (brown).
    • WB: 蛋白质免疫印迹;
    • IHC-P: 石蜡切片样本的免疫组织化学;
    • IHC-F: 冰冻切片样本的免疫组织化学;
    • ICC/IF: 细胞免疫荧光;
    • IF-Tissue: 组织免疫荧光;
    • mIHC: 多重荧光免疫组化;
    • IP: 免疫沉淀;
    • ChIP: 染色质免疫沉淀;
    • FC: 流式细胞术;
    • ELISA: 酶联免疫吸附试验

    • 产品详情

    • 验证图片

    • 背景信息

    • 产品资料

    描述

    4 Hydroxynonenal Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to 4 Hydroxynonenal.
    宿主

    Rabbit
    克隆性

    Polyclonal
    分子量

    Predicted band size: 0.156 kDa
    反应种属
    Species independent
    蛋白数据库

    基因 ID
    免疫原

    KLH conjugated 4-Hydroxynonenal.
    应用 & 推荐
    稀释比例
    应用 稀释比
    WB
    WB: 蛋白质免疫印迹
    		1:500-2000
    IHC-P
    IHC-P: 石蜡切片样本的免疫组织化学
    		1:100-500
    IF-Tissue
    IF-Tissue: 组织免疫荧光
    		1:100-500
    敏感性 Endogenous 纯度 affinity purified
    偶联 Non-conjugated 修饰 Unmodified
    同型 IgG  
    性状

    液体
    组分

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 & 期限

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
    运输条件

    Shipping with blue ice.
    验证图片
    ALL IHC mIHC

    • Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

    • Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

    • Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

    • Immunohistochemical analysis of paraffin-embedded human Colon cancer‌‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

    • Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

    • Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

    • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

    • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

    • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

    • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

    • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

    • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

    背景
    功能：4-Hydroxynonenal (HY-113466)：4-Hydroxynonenal (4-HNE) 是一种 α，β 不饱和羟基烯醛，是一种氧化/亚硝化应激生物标志物。4-Hydroxynonenalal 是 ALDH2 的底物和抑制剂。4-Hydroxynonenalal 可以调节许多信号传导过程，主要是通过与蛋白质，核酸和膜脂质中具有亲核官能团的共价加合物形成的。4-Hydroxynonenalal 通过线粒体在癌症中起重要作用。
    RRID
    中文名
    4-羟基壬烯醛抗体
    同用名
    4-Hydroxy-2-Nonenal; 4Hydroxynonenal; 4-Hydroxynonenal; HNE; 4HNE; 4-HNE; (E)-4-Hydroxy-2-nonenal; 2-Nonenal, 4-hydroxy-, (2E)-; (2E)-4-Hydroxy-2-nonenal; 4-Hydroxy-2(E)-nonenal
    文件资料

    COA (192 KB)

    抗体使用指南 (1083 KB)

    4 Hydroxynonenal Antibody 相关分类

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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