53BP1 抗体 (YA649)

目录号: HY-P80001 一键复制产品信息: Data Sheet SDS COA 抗体使用指南
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53BP1 Antibody (YA649) 是一个兔来源、无偶联标记、抗 53BP1 的 IgG 单克隆抗体。

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50 μL	￥1600	In-stock
100 μL	￥2600	In-stock
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MCE 顾客使用本产品发表的 1 篇科研文献

•Biochem Pharmacol. 2024 Jun:224:116234. [Abstract]

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

产品详情
验证图片
背景信息
产品资料

描述

53BP1 Antibody (YA649) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to 53BP1.

宿主

Rabbit

克隆性

Recombinant,Monoclonal

分子量

Predicted band size: 214 kDa;

Observed band size: 450 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse, Rat

蛋白数据库

Q12888

基因 ID

7158 [NCBI]

免疫原

Synthetic peptide corresponding to Human 53BP1.AA range:1-50.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
ICC/IF ICC/IF: 细胞免疫荧光	1:100-1:500
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:50-1:200
FC FC: 流式细胞术	1:50-1:100

敏感性	Endogenous	纯度	Protein A affinity purified.
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

IHC

Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using 53BP1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80001, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Bladder cancer‌ tissue using 53BP1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80001, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using 53BP1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80001, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using 53BP1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80001, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using 53BP1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80001, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using 53BP1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80001, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

背景

功能：双链断裂 (DSB) 修复蛋白参与 DNA 损伤反应、端粒动力学和抗体生成过程中的类别转换重组 (CSR) (PubMed:12364621, PubMed:17190600, PubMed:21144835, PubMed:22553214, PubMed:23333306, PubMed:27153538, PubMed:28241136, PubMed:31135337, PubMed:37696958)。 ATM 在 DNA 损伤后双链 DNA 断裂 (DSB) 的修复中发挥关键作用，它通过促进非同源末端连接 (NHEJ) 介导的 DSB 修复，并特异性地拮抗同源重组 (HR) 修复蛋白 BRCA1 的功能 (PubMed:22553214, PubMed:23333306, PubMed:23727112, PubMed:27153538, PubMed:31135337)。在 DSB 发生后，ATM 的磷酸化作用促进其与 RIF1 的相互作用，并使其从 NUDT16L1/TIRR 中解离，从而募集到 DSB 位点 (PubMed:28241136)。该蛋白通过识别并结合赖氨酸 15 位点单泛素化的组蛋白 H2A (H2AK15Ub) 和赖氨酸 20 位点二甲基化的组蛋白 H4 (H4K20me2) 而被募集到 DNA 双链断裂 (DSB) 位点，这两种组蛋白修饰均存在于 DSB 位点 (PubMed:17190600, PubMed:23760478, PubMed:27153538, PubMed:28241136)。在抗体生成过程中，免疫球蛋白类别转换重组 (CSR) 是必需的，而 CSR 过程涉及 DNA 双链断裂的产生 (PubMed:23345425)。该蛋白参与 CSR 过程中 DNA 断裂末端的修复和定向 (基于相似性)。相反，它并非经典非同源末端连接 (NHEJ) 和 V (D) J 重组所必需 (基于相似性)。通过与 PAXIP1 相互作用促进功能异常端粒的非同源末端连接 (PubMed:23727112)

亚细胞定位：细胞核；染色体；染色体、着丝粒、动粒

亚基：同源寡聚体 (PubMed:16294047、PubMed:23345425、PubMed:23760478)。与 p53/TP53 相互作用 (通过中心结构域)(PubMed:11877378、PubMed:12110597)。与 DCLRE1C 相互作用 (PubMed:15574327)。与组蛋白 H2AX 相互作用，且该相互作用需要 H2AX 在 Ser-139 位点磷酸化 (PubMed:12607005)。与在 Lys-20 位点二甲基化的组蛋白 H4 (H4K20me2) 相互作用 (PubMed:17190600)。对含有单甲基化赖氨酸 20 (H4K20me1) 的组蛋白 H4 亲和力低 (PubMed:17190600)。不与含有未甲基化或三甲基化赖氨酸 20 (H4K20me3) 的组蛋白 H4 结合 (PubMed:17190600)。对赖氨酸 79 二甲基化的组蛋白 H3 亲和力低 (PubMed:15525939)。对赖氨酸 79 单甲基化的组蛋白 H3 (体外) 亲和力极低 (PubMed:15525939)。不与未甲基化的组蛋白 H3 结合 (PubMed:15525939)。与赖氨酸 15 位点单泛素化的组蛋白 H2A (H2AK15Ub) 相互作用 (PubMed:23760478)。与 PWWP3A/EXPAND1 相互作用 (PubMed:20347427)。与 CHEK2 相互作用；响应红外线调节 CHEK2 在 Thr68 位点的磷酸化 (PubMed:12364621)。与 MSL1 相互作用；这种相互作用可能是 MSL1 DNA 修复活性所必需的，但并非组蛋白乙酰转移酶活性所必需的 (PubMed:19650074)。(被 ATM 磷酸化后) 与 RIF1 相互作用 (PubMed:23333306, PubMed:23727112, PubMed:28241136)。通过 Tudor 样结构域与 NUDT16L1/TIRR 相互作用；这种相互作用掩盖了 Tudor 样结构域，从而阻止其募集到染色质上 (PubMed:28241136)。与 PAXIP1 相互作用 (PubMed:23727112)。与 SHLD2 相互作用 (PubMed:29789392)。磷酸化后与 TOPBP1 相互作用 (PubMed:31135337)。与 GFI1 相互作用；促进 PRMT1 介导的甲基化 (PubMed:29651020)。与磷酸化的 DYNLL1 相互作用；特异性结合在 Ser-88 位点磷酸化的 DYNLL1，并促进其募集到双链断裂 (DSB) 位点 (PubMed:37696958)。

RRID

AB_3102018

反应种属数据库

Entrez Gene: 7158 Human ; 27223 Mouse ; 296099 Rat

SwissProt: Q12888 Human ; P70399 Mouse

OMIM: 605230 Human

研究领域

Epigenetics and Nuclear Signaling

中文名

53BP1 抗体

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (194 KB)

抗体使用指南 (1083 KB)

53BP1 Antibody (YA649) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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53BP1 抗体 (YA649)

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MCE 顾客使用本产品发表的 1 篇科研文献

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MCE 顾客使用本产品发表的 1 篇科研文献

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53BP1 Antibody (YA649) 相关分类

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