2. 抗体
  3. 一抗
  4. 单克隆抗体 重组抗体
  5. CD68 抗体 (YA6052)

CD68 Antibody (YA6052) 是一个兔来源、无偶联标记、抗 CD68 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
20 μL ￥1350 In-stock
50 μL ￥1880 In-stock
100 μL ￥3000 In-stock
250 μL   询价  

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Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

CD68 Antibody (YA6052) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD68.
宿主

Rabbit
克隆性

Monoclonal
分子量
Predicted band size: 37 kDa;
Observed band size: 100 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Mouse, Rat
蛋白数据库
基因 ID
应用 & 推荐
稀释比例
应用 稀释比
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:500-1:2000
WB
WB: 蛋白质免疫印迹
1:2000-1:10000
ICC/IF
ICC/IF: 细胞免疫荧光
1:200-1:1000
ELISA
ELISA: 酶联免疫吸附试验
1:5000-1:20000
IP
IP: 免疫沉淀
1:50-1:200
纯度 Protein A 偶联 Non-conjugated
修饰 Unmodified 同型 IgG/Kappa
性状

液体
组分

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
IHC

  • Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD68 Antibody (YA6052). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86360, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

背景
功能：CD68 可能在组织巨噬细胞的吞噬活动中发挥作用，包括细胞内溶酶体代谢以及细胞外细胞间和细胞-病原体相互作用。它能与组织和器官特异性凝集素或选择素结合，从而使巨噬细胞亚群归巢至特定部位。CD68 从内体和溶酶体快速循环至质膜，可能使巨噬细胞能够爬过含有选择素的底物或其他细胞。
亚细胞定位：细胞膜；单次跨膜 I 型膜蛋白；内体膜；单次跨膜 I 型膜蛋白；溶酶体膜；单次跨膜 I 型膜蛋白
表达水平：
组织特异性：在血液单核细胞和组织巨噬细胞中高表达。也在淋巴细胞、成纤维细胞和内皮细胞中表达。在许多肿瘤细胞系中表达，这可能使它们能够附着于血管内皮上的选择素，从而促进其扩散至继发部位。
异构体 & 翻译后修饰：P34810 有 3 种异构体：P34810-1：37408 Da (预测值)；P34810-2：31738 Da (预测值)；P34810-3：34654 Da (预测值)。
N- 和 O-糖基化
RRID
中文名
CD68 抗体 (YA6052)
同用名
CD68; Macrosialin; Gp110; CD68
文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

CD68 Antibody (YA6052) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD68 Antibody (YA6052)
目录号：
HY-P86360
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