1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. CSF-1R 抗体 (YA1299)

CSF-1R Antibody (YA1299) 是一个兔来源、无偶联标记、抗 CSF-1R 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥493 In-stock
50 μL ¥1281 In-stock
100 μL ¥2100 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

CSF-1R Antibody (YA1299) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CSF-1R.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量
Predicted band size: 109 kDa;
Observed band size:109 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human
蛋白数据库
基因 ID
免疫原

A synthesized peptide derived from human CSF1R

应用 & 推荐
稀释比例
应用 稀释比
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:100-1:200
敏感性 Endogenous 纯度 Protein A
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL IHC mIHC
  • Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using CSF-1R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81554, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using CSF-1R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81554, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using CSF-1R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81554, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using CSF-1R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81554, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using CSF-1R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81554, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using CSF-1R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81554, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using CSF-1R antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81554, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using CSF-1R antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81554, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using CSF-1R antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81554, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using CSF-1R antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81554, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using CSF-1R antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81554, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using CSF-1R antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81554, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

背景
功能:酪氨酸蛋白激酶,作为 CSF1 和 IL-34 的细胞表面受体,在造血前体细胞 (尤其是单核吞噬细胞,如巨噬细胞和单核细胞) 的存活、增殖和分化调控中发挥着至关重要的作用。它促进 IL-34 和 CSF1 刺激下促炎趋化因子的释放,从而在先天免疫和炎症过程中发挥重要作用。它在破骨细胞增殖和分化、骨吸收的调控中发挥重要作用,并且是正常骨骼和牙齿发育所必需的。它对正常的男性和女性生育能力以及妊娠期间乳腺导管和腺泡结构的正常发育至关重要。它促进肌动蛋白细胞骨架的重组,调节膜皱褶的形成、细胞黏附和细胞迁移,并促进癌细胞侵袭。配体结合后,该蛋白激活多种信号通路,包括 ERK1/2 和 JNK 通路 (PubMed:20504948, PubMed:30982609)。它能磷酸化 PIK3R1、PLCG2、GRB2、SLA2 和 CBL。PLCG2 的激活导致细胞信号分子二酰甘油和 1,4,5-三磷酸肌醇的生成,进而激活蛋白激酶 C 家族成员,尤其是 PRKCD。PIK3R1 是磷脂酰肌醇 3-激酶的调节亚基,其磷酸化可激活 AKT1 信号通路。活化的 CSF1R 还能介导 MAP 激酶 MAPK1/ERK2 和/或 MAPK3/ERK1 以及 SRC 家族激酶 SRC、FYN 和 YES1 的活化。活化的 CSF1R 通过两种途径传递信号:一是通过与其胞内结构域中磷酸化酪氨酸残基直接相互作用的蛋白质;二是通过衔接蛋白,例如 GRB2。CSF1R 促进 STAT 家族成员 STAT3、STAT5A 和/或 STAT5B 的活化;二是促进 SHC1 和 INPP5D/SHIP-1 的酪氨酸磷酸化。受体信号传导受蛋白磷酸酶 (例如 INPP5D/SHIP-1) 的下调,这些磷酸酶可使受体及其下游效应分子去磷酸化;此外,活化受体的快速内化也会下调受体信号传导。在中枢神经系统中,CSF1R 可能参与小胶质细胞和巨噬细胞的发育 (PubMed:30982608)。
亚细胞定位:细胞膜;单次跨膜 I 型膜蛋白
表达水平:
组织特异性:在骨髓和分化的血液单核细胞中表达

诱导:受糖皮质激素上调
异构体 & 翻译后修饰:P07333 有两种异构体:P07333-1:107984 Da (预测值);P07333-2:33248 Da (预测值)。
P07333 在与 CSF1 或 IL34 结合后发生自磷酸化 (PubMed:20489731, PubMed:23408870, PubMed:24336230)。Tyr-561 位点的磷酸化对于通过泛素化、内吞和降解进行正常的信号下调至关重要。Tyr-561 和 Tyr-809 位点的磷酸化对于与 SRC 家族成员 (包括 FYN、YES1 和 SRC) 的相互作用以及这些蛋白激酶的后续激活至关重要。Tyr-699 和 Tyr-923 位点的磷酸化对于与 GRB2 的相互作用至关重要。 Tyr-723 位点的磷酸化对于与 PIK3R1 的相互作用至关重要。Tyr-708 位点的磷酸化对于受体的正常降解至关重要。Tyr-723 和 Tyr-809 位点的磷酸化对于与 PLCG2 的相互作用至关重要。Tyr-969 位点的磷酸化对于与 CBL 的相互作用至关重要。PTPN2 介导的去磷酸化负调控下游信号传导和巨噬细胞分化;泛素化。自身磷酸化后迅速发生多聚泛素化,导致其降解。
亚基:与 INPPL1/SHIP2 和 THOC5 相互作用 (基于相似性)。单体。同源二聚体。与 CSF1 和 IL34 相互作用。与二聚体 CSF1 或 IL34 相互作用导致受体同源二聚化。与 PLCG2 相互作用 (酪氨酸磷酸化)(通过 SH2 结构域)。与 PIK3R1 相互作用 (酪氨酸磷酸化)(通过 SH2 结构域)。与 FYN、YES1 和 SRC 相互作用 (酪氨酸磷酸化)(通过 SH2 结构域)。与 CBL、GRB2 和 SLA2 相互作用 (酪氨酸磷酸化)。
RRID
反应种属数据库
研究领域

Cell biology

中文名
CSF-1R 抗体 (YA1299)
同用名
CSF-1R, M-CSF-R, CD115, CSF1R, FMS
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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