1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. Gli1 抗体 (YA414)

Gli1 Antibody (YA414) 是一个兔来源、无偶联标记、抗 Gli1 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥493 In-stock
50 μL ¥1281 In-stock
100 μL ¥2100 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Gli1 Antibody (YA414) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gli1.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量
Predicted band size: 118 kDa;
Observed band size: 150 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Mouse, Rat
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human Gli1.AA range:38-82.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:200
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB IHC-P
  • Western blot analysis of extracts from K-562(lane 2(20μg),Hela(lane 3(20μg) and HEK293(lane 4(20μg) using Gli1(HY-P80143 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:作为转录激活因子 (PubMed:10806483, PubMed:19706761, PubMed:19878745, PubMed:24076122, PubMed:24217340, PubMed:24311597),可与 DNA 共有序列 5'-GACCACCCA-3'结合 (PubMed:2105456, PubMed:24217340, PubMed:8378770)。在正常发育过程中调控特定基因的转录 (PubMed:19706761)。参与颅面发育、指 (趾) 发育以及中枢神经系统和消化道的发育。介导 SHH 信号通路 (PubMed:19706761, PubMed:28973407)。通过其在 SHH 信号通路中的作用,参与细胞增殖和分化 (PubMed:11238441, PubMed:28973407);作为转录激活因子,但激活的基因集与异构体 1 不同。与异构体 1 不同,激活 CD24 的表达。介导 SHH 信号通路。促进癌细胞迁移
亚细胞定位:细胞质;细胞核;细胞质;细胞核
表达水平:
组织特异性:在睾丸中检测到 (蛋白水平) (PubMed:2105456) 。睾丸、子宫肌层和输卵管中也有表达。脑组织中也有表达,在小脑、视神经和嗅束中的表达最高 (PubMed:19878745) 。异构体 1 在脑、脾、胰腺、肝脏、肾脏和胎盘中均有检测到;异构体 2 在这些组织中未检测到 (PubMed:19706761) 。

诱导:胶质母细胞瘤细胞中异构体1和异构体2均发生扩增。
亚基:与 STK36 相互作用 (PubMed:10806483)。与 ZIC1 相互作用;该相互作用增强转录激活 (PubMed:11238441)。
RRID
反应种属数据库
研究领域

Epigenetics and Nuclear Signaling

中文名
Gli1 抗体 (YA414)
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Gli1 Antibody (YA414)
目录号:
HY-P80142
需求量: