2. 抗体
  3. 一抗
  4. 单克隆抗体 重组抗体
  5. Glucagon 抗体 (YA921)

Glucagon Antibody (YA921) 是一个兔来源、无偶联标记、抗 Glucagon 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ￥567 In-stock
50 μL ￥1470 In-stock
100 μL ￥2362 In-stock
250 μL   询价  

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Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Glucagon Antibody (YA921) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucagon.
宿主

Rabbit
克隆性

Monoclonal,Recombinant
分子量
Predicted band size: 21 kDa;
Observed band size: 21 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

A synthesized peptide derived from human Glucagon aa53-73.
应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
敏感性 Endogenous 纯度 Affinity Purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体
组分

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL IHC mIHC

  • Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using Glucagon antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81242, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using Glucagon antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81242, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using Glucagon antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81242, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using Glucagon antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81242, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Pancreas tissue using Glucagon antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81242, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Pancreas tissue using Glucagon antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81242, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Pancreas tissue using Glucagon antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81242, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Pancreas tissue using Glucagon antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81242, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

背景
功能：在葡萄糖代谢和稳态中发挥关键作用。通过增加糖异生和减少糖酵解来调节血糖。作为胰岛素的拮抗激素，在胰岛素诱导的低血糖时升高血糖水平。在糖尿病高血糖状态的启动和维持中发挥重要作用；是葡萄糖依赖性胰岛素释放的强效刺激剂。还能刺激 IL-6 诱导的胰岛素释放 (PubMed:22037645)。对胃动力和血浆胰高血糖素水平的抑制起重要作用。可能参与抑制饱腹感和刺激外周组织葡萄糖利用，且该作用独立于胰岛素。对肠上皮细胞具有促生长作用。也可能通过影响 LH、TSH、CRH、催产素和血管加压素的分泌来调节下丘脑-垂体-肾上腺轴 (HPA 轴)。通过刺激胰岛新生和胰岛β细胞增殖来增加胰岛质量。抑制β细胞凋亡 (可能)；刺激肠道生长并上调小肠绒毛高度，同时伴有隐窝细胞增殖增加和肠上皮细胞凋亡减少。从胃到结肠的胃肠道是 GLP-2 的主要作用靶点。在营养稳态中发挥关键作用，通过增强胃肠功能来促进营养吸收，并增加营养物质的清除。刺激肠道葡萄糖转运并降低黏膜通透性；显著减少食物摄入。抑制人体胃排空。胃排空抑制可能导致胃扩张增加，从而产生饱腹感，进而增强饱腹感；可能调节胃酸分泌和胃幽门十二指肠活动。可能在生命早期肠黏膜生长中发挥重要作用。
亚细胞定位：分泌的；分泌的
表达水平：
组织特异性：由胰岛 A 细胞分泌；由胰岛 A 细胞分泌 (PubMed:22037645) 。由遍布整个胃肠道的肠内分泌 L 细胞分泌 (PubMed:22037645) 。也由脑内某些神经元分泌；由遍布整个胃肠道的肠内分泌细胞分泌。也由脑内某些神经元分泌；由遍布整个胃肠道的肠内分泌细胞分泌；由遍布整个胃肠道的肠内分泌细胞分泌

诱导：低血糖刺激其释放，高血糖、胰岛素和生长抑素抑制其释放；IL-6 可增加胰岛 α 细胞的产生；营养摄入可诱导其产生
反应种属数据库

Entrez Gene: 2641 Human ; 14526 Mouse ;

SwissProt: P01275 Human ; P55095 Mouse ; P06883 Rat

OMIM: 138030 Human

中文名
Glucagon 抗体
同用名
GCG antibody; Glucagon
文件资料

COA (194 KB)

抗体使用指南 (1083 KB)

Glucagon Antibody (YA921) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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