2. 抗体
  3. 一抗
  4. 单克隆抗体 重组抗体 流式抗体
  5. JNK2 抗体 (YA326)

JNK2 Antibody (YA326) 是一个兔来源、无偶联标记、抗 JNK2 的 IgG 单克隆抗体。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 μL ￥493 In-stock
50 μL ￥1281 In-stock
100 μL ￥2100 In-stock
250 μL   询价  

* Please select Quantity before adding items.

实现从蛋白分离到检测的高效衔接！

加购更优惠，一站式完成电泳实验！
Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

JNK2 Antibody (YA326) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to JNK2.
宿主

Rabbit
克隆性

Recombinant,Monoclonal
分子量
Predicted band size: 48/44 kDa;
Observed band size: 48/44 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human JNK2.AA range:341-394.
应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:1000-1:5000
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:200
FC
FC: 流式细胞术
1:50-1:100
IP
IP: 免疫沉淀
Use at an assay dependent concentration.
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体
组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
IHC

  • Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using JNK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80199, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using JNK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80199, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using JNK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80199, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using JNK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80199, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using JNK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80199, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using JNK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80199, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

背景
功能：丝氨酸/苏氨酸蛋白激酶参与多种过程，例如细胞增殖、分化、迁移、转化和程序性细胞死亡 (PubMed:10376527, PubMed:15805466, PubMed:17525747, PubMed:19675674, PubMed:20595622, PubMed:21364637, PubMed:22441692, PubMed:34048572)。细胞外刺激，例如促炎细胞因子或物理应激，会激活应激激活蛋白激酶/c-Jun N 端激酶 (SAP/JNK) 信号通路。在该级联反应中，两种双特异性激酶 MAP2K4/MKK4 和 MAP2K7/MKK7 磷酸化并激活 MAPK9/JNK2 (PubMed:10376527, PubMed:15805466, PubMed:17525747, PubMed:19675674, PubMed:20595622, PubMed:21364637, PubMed:22441692, PubMed:34048572)。反过来，MAPK9/JNK2 磷酸化多种转录因子，主要是 AP-1 的组成部分，例如 JUN 和 ATF2，从而调节 AP-1 的转录活性 (PubMed:10376527)。在氧化或核糖体毒性应激下，MAPK8 和 MAPK9 通过磷酸化并灭活 RNA 聚合酶 1 特异性转录起始因子 RRN3 来抑制 rRNA 合成 (PubMed:15805466)。MAPK8 和 MAPK9 通过磷酸化关键调控因子 (包括 TP53 和 YAP1) 来促进应激细胞凋亡 (PubMed:17525747, PubMed:21364637)。在 T 细胞中，MAPK8 和 MAPK9 是 T 辅助细胞极化分化为 Th1 细胞所必需的 (PubMed:19290929)。T 细胞受体 (TCR) 刺激后，MAPK8 和 MAPK9 被 CARMA1、BCL10、MAP2K7 和 MAP3K7/TAK1 激活，从而调节 JUN 蛋白水平 (PubMed:19290929)。在渗透压应激诱导的上皮紧密连接破坏中发挥重要作用 (PubMed:20595622)。激活后，促进β-catenin/CTNNB1 降解并抑制经典 Wnt 信号通路 (PubMed:19675674)。也参与螺旋神经节神经元的神经突生长 (基于相似性)。磷酸化 CLOCK-BMAL1 异二聚体，并在生物钟调控中发挥作用 (PubMed:22441692)。磷酸化 POU5F1，导致 POU5F1 转录活性受到抑制并增强其蛋白酶体降解 (基于相似性)。响应活性氧 (ROS) 磷酸化 ALKBH5，促进 ALKBH5 的 SUMO 化和失活 (PubMed:34048572)。 MAPK9 亚型表现出不同的结合模式：α1 和α2 优先结合 JUN，而β1 和β2 结合 ATF2。然而，结合与磷酸化之间没有相关性，所有亚型的磷酸化效率大致相同。JUNB 不是 JNK2 α2 的底物，而 JUND 与其结合较弱。
亚细胞定位：细胞质；细胞核
亚基：与 NFATC4 相互作用 (PubMed:17875713)。与 ATF7 相互作用；该相互作用不使 ATF7 磷酸化，但可作为 ATF7 相关伴侣 (如 JUN) 的停泊位点 (PubMed:10376527)。与 BCL10 相互作用 (PubMed:17189706)。
RRID
反应种属数据库

Entrez Gene: 5601 Human ; 26420 Mouse ; 50658 Rat

SwissProt: P45984 Human ; Q9WTU6 Mouse ; P49186 Rat

OMIM: 602896 Human

研究领域

Signal Transduction
中文名
JNK2 抗体
文件资料

COA (194 KB)

抗体使用指南 (1083 KB)

JNK2 Antibody (YA326) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
JNK2 Antibody (YA326)
目录号：
HY-P80199
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z