2. 抗体
  3. 一抗
  4. 多克隆抗体 流式抗体
  5. Substance P 抗体

Substance P Antibody 是一个兔来源、无偶联标记、抗 Substance P 的 IgG 多克隆抗体。

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规格 价格 是否有货 数量
20 μL ￥846 In-stock
50 μL ￥1220 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

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Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Substance P Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Substance P.
宿主

Rabbit
克隆性

Polyclonal
分子量
Predicted band size: 1.4/13-15 kDa;
Observed band size: 20 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse, Rat, Guinea Pig 预测反应种属: Pig,Cow,Horse,Rabbit,Sheep
请注意：预测反应种属仅供参考，不作为质保凭证。
蛋白数据库
基因 ID
免疫原

KLH conjugated synthetic peptide derived from human Substance P (RPKPQQFFGLM): 58-68/129
应用 & 推荐
稀释比例
应用 稀释比
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:100-500
IHC-F
IHC-F: 冰冻切片样本的免疫组织化学
1:100-500
FC
FC: 流式细胞术
1μg/Test
ICC/IF
ICC/IF: 细胞免疫荧光
1:100
IF-Tissue
IF-Tissue: 组织免疫荧光
1:100-500
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体
组分

1.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
2.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Please refer to the lot-specific COA for specific buffer information.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL IHC mIHC

  • Immunohistochemical analysis of paraffin-embedded human Prostate Cancer‌ tissue using Substance P antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Substance P antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Substance P antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using Substance P antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Substance P antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using Substance P antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using Substance P antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using Substance P antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using Substance P antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Substance P antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Substance P antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Substance P antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81130, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

背景
功能：速激肽是活性肽，能够兴奋神经元，诱发行为反应，是强效的血管舒张剂和促分泌剂，并能 (直接或间接地) 收缩多种平滑肌。
亚细胞定位：分泌物
异构体 & 翻译后修饰：P20366 有 4 种异构体：P20366-1：15003 Da (预测值)；P20366-2：13036 Da (预测值)；P20366-3：13343 Da (预测值)；P20366-4：11376 Da (预测值)。
P 物质在体外可被脯氨酰内肽酶 FAP (丝氨酸蛋白酶) 在 Pro-59 位点裂解 (PubMed:21314817)。P 物质也可被血管紧张素转换酶 (ACE) 和脑啡肽酶 (MME) 裂解和降解 (PubMed:6208535)。
RRID
反应种属数据库

Entrez Gene: 6863 Human ; 21333 Mouse ; 24806 Rat

SwissProt: P20366 Human ; P41539 Mouse ; P06767 Rat

OMIM: 162320 Human

中文名
P物质抗体
同用名
Hs.2563; Neurokinin 1; Neurokinin 2; Neurokinin A; Neurokinin alpha; Neuromedin L; Neuropeptide gamma; NKA; NKNA; PPT; Protachykinin 1 precursor; Substance K; Substance P; TAC1; TAC2; Tachykinin 1; Tachykinin 2; Tachykinin precursor 1; Tachykinin1; TACI; TKN1_HUMAN.
文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

Substance P Antibody 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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