2. Others
  3. Fluorescent Dye
  4. APC-Cy7

APC-Cy7 是一种在流式细胞术中广泛使用的串联荧光染料。APC-Cy7 的核心是一个串联复合物：由别藻蓝蛋白 (APC) 作为能量供体，Cy7 作为能量受体。APC-Cy7 可用于多色流式分析，因为它与 FITC、PE 等其他常见荧光染料的发射光谱重叠极小，有效减少了荧光补偿 (Ex/Em = 650 nm/778 nm)。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

APC-Cy7

APC-Cy7 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货
1 mg ￥4300
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5 mg 询价 询问价格 & 货期

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APC-Cy7 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

APC-Cy7 is an APC-tandem fluorochrome (allophycocyanin-cyanine 7) and degradable fluorochrome, with maximum absorption at ~650 nm, peak emission at 767 nm when excited by a 633 nm red laser, and cell-dependent degradation. APC-Cy7 undergoes time-dependent degradation (decoupling) when bound to peripheral blood leukocytes, leading to nonspecific APC channel signal, with degradation cell type-dependent. APC-Cy7 shows reduced degradation when immunolabeled cells have inhibited metabolic activity or are incubated with vitamin C. APC-Cy7 serves as a tandem fluorochrome in multiparametric flow cytometry for detection of leukocyte surface proteins when conjugated to specific antibodies (Ex/Em = 650 nm/778 nm)[1].

体外研究
(In Vitro)

APC-Cy7 (0-60 min) 偶联抗 CD45 抗体后，在人外周血淋巴细胞、单核细胞和多形核白细胞中会发生时间依赖性降解 (解偶联)，其中淋巴细胞的解偶联率从 0 min 时的约 5%上升至 30 min 时的约 19%；而结合抗体的 CompBeads 除非与未标记细胞共同孵育，否则不会发生解偶联[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

APC-Cy7 相关抗体:
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

APC-Cy7 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

APC-Cy7Fluorescent Dyehuman peripheral blood granulocytesPRICompBeadsCD3-positive T lymphocyteshuman peripheral blood lymphocytesJeKo human B cell linesGRANTAmonocytesperipheral blood leukocytespolymorphonuclear leukocytesInhibitorinhibitorinhibit

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产品名称：
APC-Cy7
目录号：
HY-D3330
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