Calcein (solution)  (Synonyms: Fluorexon (solution))

Calcein (solution) (Fluorexon (solution)) is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems^[1][2].
Solvent and concentration: DMSO: 2 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

描述：Calcein 是一种发出黄绿色荧光的荧光探针，可用于测定不稳定铁离子池以及测量 Fe(III) 与配体的结合亲和力^[3]。
1.溶液配制^[3]
1.1 工作液配制
用 PBS 或细胞培养基稀释至 0.5 μM。
注意：工作液应立即配制并使用，避光保存。

2. 细胞染色
2.1 将细胞 (10⁶/mL) 与 20 μM Fe(II) 在 37°C 下孵育 2 小时。 2.2 离心 (1000 rpm；5 分钟) 收集细胞，并将 Calcein (0.5 μM) 通过显微注射直接注射到细胞中进行染色 (5-15 mins)。 2.3 用 D'hanks 缓冲液 (pH 7.4) 洗涤细胞两次。 2.4 使用流式细胞术和共聚焦激光扫描显微镜监测荧光。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

622.53

C₃₀H₂₆N₂O₁₃

1461-15-0

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203.  [Content Brief]

  [2]. Shao B, et al. Caffeic Acid Phenyl Ester (CAPE) Protects against Iron-Mediated Cellular DNA Damage through Its Strong Iron-Binding Ability and High Lipophilicity. Antioxidants (Basel). 2021 May 18;10(5):798.  [Content Brief]

  [3]. Maherani B, et al., Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids. Biochimie. 2013 Nov;95(11):2018-33.  [Content Brief]