Diphtheria toxin CRM197 

Diphtheria toxin CRM197, a nontoxic variant of diphtheria toxin, has a single mutation (Glycine to Glutamate substitution at position 52) that eliminates its toxicity. CRM197 is widely used as a carrier protein in conjugate vaccines^[1].

Diphtheria toxin CRM197 is an enzymatically inactive and nontoxic form of diphtheria toxin that contains a single amino acid substitution (G52E). Being naturally nontoxic, CRM197 is an ideal carrier protein for conjugate vaccines against encapsulated bacteria and is currently used to vaccinate children globally against Haemophilus influenzae, pneumococcus, and meningococcus^[1][2].
Diphtheria toxin, a single polypeptide secreted by lysogenic strains of Corynebacterium diphtheria, causes the disease diphtheria in humans by gaining entry into the cytoplasm of cells and inhibiting protein synthesis^[3][4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

92092-36-9

液体

Colorless to light yellow

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ravi P N Mishra, et al. Structural and Immunological Characterization of E. coli Derived Recombinant CRM 197 Protein Used as Carrier in Conjugate Vaccines. Biosci Rep. 2018 Sep 25;38(5):BSR20180238.  [Content Brief]

  [2]. Enrico Malito, et al. Structural Basis for Lack of Toxicity of the Diphtheria Toxin Mutant CRM197. Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5229-34.  [Content Brief]

  [3]. C E Bell, et al. Crystal Structure of Diphtheria Toxin Bound to Nicotinamide Adenine Dinucleotide. Biochemistry. 1996 Jan 30;35(4):1137-49.  [Content Brief]

  [4]. Ranjit Sah, et al. Diphtheria. N Engl J Med. 2019 Sep 26;381(13):1267.  [Content Brief]