2. Immunology/Inflammation
  3. Toll-like Receptor (TLR) IFNAR Interleukin Related
  4. ENDO12

ENDO12 是一种 Munc13-4-STX7 蛋白复合物抑制剂，对 STX7 的 Kd 值为 2.7 µM。ENDO12 可阻断 Munc13-4-STX7 的相互作用。ENDO12 可抑制内溶酶体通量、内溶酶体货物降解、中性粒细胞中的细胞外信号调节激酶信号通路、浆细胞样树突状细胞中的 IFN 调节因子信号通路，以及原代树突状细胞对 TLR3、TLR7、TLR9 的应答。ENDO12 可通过降低髓过氧化物酶、IL-6 和 IFNγ 的水平，减轻 CpG 诱导的全身性炎症。ENDO12 不会干扰宿主对淋巴细胞脉络丛脑膜炎病毒感染的抗病毒应答。\nENDO12 可用于全身性炎症的相关研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

ENDO12

ENDO12 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

ENDO12 相关抗体

Top Publications Citing Use of Products

查看 Toll-like Receptor (TLR) 亚型特异性产品:

查看所有亚型
TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

查看 Interleukin Related 亚型特异性产品:

查看所有亚型
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

ENDO12 is an inhibitor of the Munc13-4-STX7 protein complex, with a Kd value of 2.7 µM for STX7. ENDO12 blocks the interaction of Munc13-4-STX7. ENDO12 inhibits endolysosomal flux, endolysosomal cargo degradation, the extracellular signal-regulated kinase signaling pathway in neutrophils, the IFN regulatory factor signaling pathway in plasmacytoid dendritic cells, and the responses of primary dendritic cells to TLR3, TLR7, and TLR9. ENDO12 alleviates CpG-induced systemic inflammation by reducing the levels of myeloperoxidase, IL-6 and IFNγ. ENDO12 does not interfere with the host's antiviral response to lymphocytic choriomeningitis virus infection.\nENDO12 can be used in studies related to systemic inflammation[1].

IC50 & Target[1]

IL-6

 

TLR3

 

TLR9

 

TLR7

 

体外研究
(In Vitro)

ENDO12 (31.25 nM-3 μM) 与重组 STX7 结合，其 Kd 值为 2.7 µM，亲和力高于 ENDO3[1]。
ENDO12 可强效抑制 HEK-Blue hTLR9 细胞中 CpG 诱导的 TLR9 激活，其 IC50 为 1 × 10-7 M[1]。
ENDO12 (10 µM; 1 h) 可降低 RAW 264.7 小鼠巨噬细胞中 STX7 与 Munc13-4、TLR7 与 LAMP1、TLR9 与 LAMP1 的共定位水平，从而抑制内体成熟和 TLR 转运[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

ENDO12 相关抗体:

Immunofluorescence[1]

Cell Line: RAW 264.7 murine macrophages
Concentration: 10 µM
Incubation Time: 1 h
Result: Significantly decreased colocalization of STX7 with Munc13-4.
Significantly decreased colocalization of TLR7 and TLR9 with LAMP1+ organelles, indicating impaired endosomal maturation and TLR trafficking to late endolysosomes.
体内研究
(In Vivo)

ENDO12 (30 mg/kg；腹腔注射；单次；CpG 攻击前 1 小时) 可减轻小鼠体内 CpG 诱导的全身性炎症，降低血浆 IL-6、IFNγ 及 MPO 水平[1]。
ENDO12 (15 mg/kg；腹腔注射；单次；淋巴细胞脉络丛脑膜炎病毒感染前 1 小时) 不会削弱小鼠对淋巴细胞脉络丛脑膜炎病毒 (LCMV) 感染的宿主抗病毒应答，仅会使 MIP1β 和 MIP2 水平出现轻微降低[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J mice (male and female, 6-10 weeks old)[1]
Dosage: 30 mg/kg
Administration: i.p.; single dose; 1 hour before CpG challenge
Result: Significantly reduced CpG-induced plasma levels of interleukin 6 (IL-6), interferon gamma (IFNγ), and myeloperoxidase (MPO) compared to vehicle-treated controls.
Animal Model: C57BL/6J mice (male and female, 8 weeks old)[1]
Dosage: 15 mg/kg
Administration: i.p.; single dose; 1 hour before LCMV infection
Result: Did not significantly alter plasma levels of IL-6, IFNα, IFNγ, or MPO induced by LCMV infection.
Caused only a mild reduction in plasma levels of macrophage inflammatory proteins MIP1β and MIP2, while all other measured cytokines and chemokines remained unchanged compared to vehicle-treated controls.
分子量

288.32

Formula

C15H17FN4O
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

ENDO12 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ENDO12ENDO 12ENDO-12Toll-like Receptor (TLR)IFNARInterleukin RelatedInterferon-α/β receptorInterferon-alpha/beta receptor ILdendritic cellsTLR9HEK-Blue hTLR9 cellsTLR7plasmacytoid dendritic cellsMunc13-4-STX7 protein complexTLR3RAW 264.7 murine macrophagesJurkat-Dual cellsneutrophilsInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
ENDO12
目录号：
HY-183079
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z