2. NF-κB MAPK/ERK Pathway Protein Tyrosine Kinase/RTK PI3K/Akt/mTOR Apoptosis Immunology/Inflammation
  3. NF-κB p38 MAPK FAK Akt Apoptosis NO Synthase COX TNF Receptor Interleukin Related
  4. Ephemeranthol A

Ephemeranthol A 是一种菲类化合物，具有抗癌和抗炎活性。Ephemeranthol A 通过抑制 NF-κB 和 MAPK 信号通路，在巨噬细胞中发挥显著的抗炎作用。Ephemeranthol A 通过抑制 FAK/Akt 信号和 EMT 过程，诱导肺癌细胞凋亡 (apoptosis) 和抑制转移。Ephemeranthol A 可用于急慢性炎症疾病和非小细胞肺癌的研究。

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Ephemeranthol A

Ephemeranthol A Chemical Structure

CAS No. : 135545-86-7

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Ephemeranthol A 相关抗体

Top Publications Citing Use of Products

查看 NF-κB 亚型特异性产品:

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

查看 p38 MAPK 亚型特异性产品:

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

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Akt Akt1 Akt2 Akt3

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nNOS iNOS eNOS

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COX COX-1 COX-2 COX-3

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TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Ephemeranthol A is a phenanthrene compound with anticancer and anti-inflammatory activities. Ephemeranthol A exerts significant anti-inflammatory effects in macrophages by inhibiting the NF-κB and MAPK signaling pathways. Ephemeranthol A induces apoptosis and inhibits metastasis of lung cancer cells by suppressing the FAK/Akt signaling and EMT processes. Ephemeranthol A can be used for the research of acute and chronic inflammatory diseases and non-small cell lung cancer[2].

IC50 & Target[1]

NF-κB

 

iNOS

 

COX-2

 

IL-1β

 

IL-6

 

体外研究
(In Vitro)

Ephemeranthol A (6.25-50 μg/mL; 25 h) 在浓度 ≤ 25 μg/mL 时对 Raw 264.7 细胞无细胞毒性，但在 50 μg/mL 浓度下会降低细胞活力[1]。
Ephemeranthol A (6.25-50 μg/mL; 7-25 h) 强效抑制 Raw 264.7 细胞中 LPS 诱导的 NO、iNOS、COX-2、TNF-α、IL-6 和 IL-1β 生成[1]。
Ephemeranthol A (25 μg/mL; 1.5-2 h) 可阻断 Raw 264.7 细胞中 LPS 诱导的 IκB 降解，抑制 NF-κB 亚基 p50 和 p65 的核转位 [1]。
Ephemeranthol A (6.25-25 μg/mL; 1 h) 可抑制 Raw 264.7 细胞中 LPS 诱导的 p38 和 JNK 磷酸化[1]。
Ephemeranthol A (5-200 μM; 24-48 h) 以浓度和时间依赖的方式降低人非小细胞肺癌 H460 细胞的活力，其 24 h 时的 IC50 > 200 μM，48 h 时的 IC50 为 150.5 μM[2]。
Ephemeranthol A (10-100 μM; 24 h) 以浓度依赖的方式诱导人非小细胞肺癌 H460 细胞发生凋亡[2]。
Ephemeranthol A (50-100 μM; 48 h) 可通过降低 Bcl-2 水平并激活 caspase-9、caspase-3 和 PARP，在 48 h 时诱导人非小细胞肺癌 H460 细胞发生细胞凋亡[2]。
Ephemeranthol A (50-100 μM; 48 h) 可在 48 h 时抑制人非小细胞肺癌 H460 细胞中 FAK-Akt 信号通路的激活[2]。
Ephemeranthol A (5-50 μM; 48 h) 可抑制人非小细胞肺癌 H460 细胞的锚定非依赖性生长[2]。
Ephemeranthol A (10-100 μM; 24-48 h) 抑制人非小细胞肺癌 H460 细胞的迁移[2]。
Ephemeranthol A (5-100 μM; 24-48 h) 可抑制人非小细胞肺癌 H460 细胞的上皮间质转化，诱导上皮形态改变[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Ephemeranthol A 相关抗体:

Western Blot Analysis[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL
Incubation Time: 1 h pre-incubation; 6 h LPS stimulation
Result: Reduced LPS-induced iNOS protein production in Raw 264.7 cells.\n
Reduced LPS-induced COX-2 protein production in Raw 264.7 cells.

RT-PCR[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL
Incubation Time: 1 h pre-incubation; 6 h LPS stimulation
Result: Significantly decreased LPS-induced mRNA levels of TNF-α, IL-6, and IL-1β.
Reduced TNF-α and IL-6 mRNA levels to the level of the unstimulated control.

ELISA Assay[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL
Incubation Time: 1 h pre-incubation; 24 h LPS stimulation
Result: Significantly inhibited LPS-induced production of TNF-α, IL-6, and IL-1β protein in Raw 264.7 cells.

Western Blot Analysis[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL
Incubation Time: 1 h pre-incubation; 30 min or 1 h LPS stimulation
Result: Blocked LPS-induced IκB degradation at 30 min post-stimulation.
Sustained the inhibitory effect until 1 h post-stimulation.\n
Inhibited LPS-induced translocation of p50 and p65 into the nucleus at 30 min post-stimulation.

Western Blot Analysis[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL (p38, JNK phosphorylation at 10/20 min); 6.25, 12.5 and 25 μg/mL (p38 phosphorylation at 10 min)
Incubation Time: 1 h pre-incubation; 10 min or 20 min LPS stimulation (p38, JNK phosphorylation); 1 h pre-incubation, 10 min LPS stimulation (p38 phosphorylation dose-response)
Result: Reduced LPS-induced phosphorylation of p38 and JNK at 10 min and 20 min post-stimulation.
Sustained the inhibitory effect on JNK phosphorylation for 20 min.
Induced a dose-dependent reduction in LPS-induced p38 phosphorylation at 6.25, 12.5, and 25 μg/mL.

Cell Cytotoxicity Assay[2]

Cell Line: human non-small cell lung cancer H460 cells
Concentration: 5, 10, 50, 100 and 200 μM
Incubation Time: 24 h; 48 h
Result: Exhibited non-toxic to slightly toxic effects at concentrations ≤ 50 μM.
Caused significant cell viability reduction at 100 and 200 μM at both 24 and 48 h.
Reached an IC50 of > 200 μM at 24 h and 150.5 μM at 48 h.

Apoptosis Analysis[2]

Cell Line: human non-small cell lung cancer H460 cells
Concentration: 10, 50 and 100 μM
Incubation Time: 24 h
Result: Induced concentration-dependent increases in apoptotic cell number.
Resulted in a significant increase to ~17% apoptotic nuclei at 100 μM.
Kept necrotic cell numbers minimal across all concentrations.

Western Blot Analysis[2]

Cell Line: human non-small cell lung cancer H460 cells
Concentration: 50, 100 μM
Incubation Time: 48 h
Result: Increased cleaved PARP levels 1.98-fold (50 μM) and 2.33-fold (100 μM).
Increased cleaved caspase-9 levels 1.52-fold (50 μM) and 1.73-fold (100 μM).
Increased cleaved caspase-3 levels 4.78-fold (100 μM).
Reduced Bcl-2 levels to 0.63-fold (100 μM).
Showed no significant effects on Mcl-1 or Bax levels.\nReduced the p-FAK/total FAK ratio to 0.82-fold (50 μM) and 0.59-fold (100 μM).
Reduced the p-Akt/total Akt ratio to 0.52-fold (100 μM).
Kept total FAK and total Akt levels largely unchanged.

Cell Migration Assay[2]

Cell Line: human non-small cell lung cancer H460 cells
Concentration: 10, 50 and 100 μM
Incubation Time: 24 h; 48 h
Result: Showed no significant effect on wound closure at 24 h.
Reduced wound closure to 33.77% (50 μM) and 25.06% (100 μM) at 48 h, compared to untreated control wound closure of 38.40%.
分子量

272.30

Formula

C16H16O4
CAS 号
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Ephemeranthol A 相关分类

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  • Do most proteins show cross-species activity?

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Keywords:

Ephemeranthol A135545-86-7NF-κBp38 MAPKFAKAktApoptosisNO SynthaseCOXTNF ReceptorInterleukin RelatedNuclear factor-κBNuclear factor-kappaBPTK2 protein tyrosine kinase 2PTK2Focal adhesion kinasePKBProtein kinase BNitric oxide synthasesNOSCyclooxygenaseTumor Necrosis Factor ReceptorTNFRILJNKnon-small cell lung cancer cellsERKiNOSmacrophagesnon-small cell lung cancerCOX-2Raw 264.7 cellsInhibitorinhibitorinhibit

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