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  3. Fluorescent Dye
  4. Fluorescein-12-dGTP

Fluorescein-12-dGTP 是一种荧光素标记的脱氧鸟苷三磷酸。Fluorescein-12-dGTP 可掺入 SNP 位点的互补等位基因特异性引物中，通过 DNA-PFP 静电相互作用实现与阳离子共轭聚合物 PFP 的 FRET。针对纯合 G/G SNP 基因型，Fluorescein-12-dGTP 可掺入 G 等位基因特异性引物，从而实现基因型区分。Fluorescein-12-dGTP 可用作均相溶液法 SNP 和 FRET 基因分型检测的荧光标记核苷酸 (Ex/Em = 498/517 nm)。

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Fluorescein-12-dGTP

Fluorescein-12-dGTP Chemical Structure

CAS No. : 936107-90-3

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Fluorescein-12-dGTP 相关抗体

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  • 生物活性

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  • 参考文献

生物活性

Fluorescein-12-dGTP is a fluorescein-labeled deoxyguanosine triphosphate. Fluorescein-12-dGTP incorporates into complementary allele-specific primers at SNP sites, enabling FRET with cationic conjugated polymer PFP via DNA-PFP electrostatic interactions. Fluorescein-12-dGTP incorporates into G-allele-specific primers for homozygous G/G SNP genotypes to enable genotype discrimination. Fluorescein-12-dGTP serves as a fluorescently labeled nucleotide for homogeneous solution-based SNP and FRET genotyping assays (Ex/Em = 498/517 nm)[1].

体外研究
(In Vitro)

Fluorescein-12-dGTP (2 μM; 60 cycles of denaturation/annealing) 可通过单碱基延伸和 PFP 介导的 FRET 技术从人类基因组 DNA 中清晰区分 rs2241715 G→T SNP 基因型 (纯合 G/G、杂合 G/T、纯合 T/T)，基因型特异性 FRET 比值范围为 0.05 至 0.38[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Fluorescein-12-dGTP 相关抗体:
分子量

1030.71

Formula

C41H41N6O20P3
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Fluorescein-12-dGTP 相关分类

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Keywords:

Fluorescein-12-dGTP936107-90-3Fluorescent Dyehuman genomic DNAdeoxyguanosine triphosphateSNP genotyping assaysSNP sitesFRET donor/acceptor partnerhomozygous G/G SNP genotypescationic conjugated polymer PFPDNA-PFP electrostatic interactionsG-allele-specific primerssingle-base extension reactionsInhibitorinhibitorinhibit

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