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  3. Biochemical Assay Reagents
  4. GALA-chol

GALA-chol 是一种胆固醇偶联的 pH 响应型融合肽，可辅助递送。GALA-chol 可增强 siRNA RET/PTC1-SQ 纳米颗粒的细胞内吞作用，抑制细胞活力，并在酸性溶酶体环境下发生 pH 响应型电荷转换，从而促进小细胞外囊泡 (sEV) cargo 的溶酶体逃逸。GALA-chol 锚定于 sEV 膜上，可维持 sEV 的结构完整性及固有归巢活性。GALA-chol 可用于辅助递送的相关研究。

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Custom Peptide Synthesis

GALA-chol

GALA-chol Chemical Structure

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GALA-chol 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

GALA-chol is a cholesterol-conjugated pH-responsive fusion peptide that can serve as a delivery adjuvant. GALA-chol enhances the endocytosis of siRNA RET/PTC1-SQ nanoparticles, inhibits cell viability, and undergoes pH-responsive charge conversion in the acidic lysosomal environment, thereby promoting lysosomal escape of small extracellular vesicle (sEV) cargo. GALA-chol anchors to the sEV membrane and maintains the structural integrity and intrinsic homing activity of sEVs. GALA-chol can be used in studies related to adjuvant delivery[1][2].

体外研究
(In Vitro)

GALA-Chol 与 siRNA RET/PTC1–SQ 组成的纳米颗粒 (GALA-Chol 占比 10%；50 nM siRNA；24-72 h) 可在 48 h 和 72 h 时使 BHP 10-3 SC 小鼠和 TPC-1 细胞的存活率降低约 20%，与 Lipofectamine 2000 联用时抑制作用增强 (约 50%)[1]。
GALA-Chol 与 siRNA RET/PTC1–SQ 组成的纳米颗粒 (GALA-Chol 占比 10%; 50 nM siRNA; 24-48 h) 可在 24 h 和 48 h 两个时间点显著抑制 BHP 10-3 SC 小鼠及 TPC-1 细胞中 RET/PTC1 基因与蛋白的表达，而不含 GALA-Chol 的 siRNA RET/PTC1-SQ 纳米颗粒则无沉默活性[1]。
GALA-Chol 与 siRNA RET/PTC1–SQ 组成的纳米颗粒 (GALA-Chol 占比 10%; 50 nM siRNA; 4 h) 可被 BHP 10-3 SCmice 细胞高效内化，而不含 GALA-Chol 的 siRNA RET/PTC1-SQ 纳米颗粒则无法穿过细胞膜[1]。
GALA-chol (0-10 μM/109 sEV particles) 功能化修饰 MCF-7 细胞来源的 sEV 后，当浓度为 8 μM/109 particles 时，向 MCF-7 细胞的细胞质 cargo 递送效率达到最高；而更高浓度下，由于空间位阻干扰，递送效率会下降[2]。
GALA-chol (8 μM/109 sEV particles; 0-10 h) 功能化的 MCF-7 来源 sEV 可在孵育 6 h 后实现货物在 MCF-7 细胞中的高效溶酶体逃逸，与未功能化的 sEV 相比，其胞质货物分布显著增加[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

GALA-chol 相关抗体:

Cell Viability Assay[1]

Cell Line: BHP 10-3 SCmice, TPC-1 (human papillary thyroid carcinoma cell lines harboring RET/PTC1 fusion oncogene)
Concentration: 10% molar ratio of GALA-Chol relative to siRNA RET/PTC1-SQ; 50 nM siRNA concentration
Incubation Time: 24 h, 48 h, 72 h
Result: Inhibited cell viability by ~10% at 24 h and ~20% at 48 h and 72 h compared to untreated cells.
Enhanced inhibition to ~50% when combined with Lipofectamine 2000.
分子量

3603.23

Formula

C170H269N35O48S
Sequence

Ac-Trp-Glu-Ala-Ala-Leu-Ala-Glu-Ala-Leu-Ala-Glu-Ala-Leu-Ala-Glu-His-Leu-Ala-Glu-Ala-Leu-Ala-Glu-Ala-Leu-Glu-Ala-Leu-Ala-Ala-{Cys(cholesterol)}-NH2
Sequence Shortening

Ac-WEAALAEALAEALAEHLAEALAEALEALAA-{Cys(cholesterol)}--NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

GALA-chol 相关分类

  • 摩尔计算器

  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GALA-cholGALA-cholesterolBiochemical Assay Reagentslysosomal escapeTPC-1 cellspapillary thyroid carcinomabreast cancerRET/PTC1 geneTGF-β1sEV membranesRET/PTC1 proteinMCF-7 cellssiRNA RET/PTC1-SQfusion peptideInhibitorinhibitorinhibit

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