2. Apoptosis
  3. Apoptosis
  4. GR-891

GR-891 是一种无环核苷 5-Fluorouracil (HY-90006) 衍生物。GR-891 可在人类癌细胞中发挥抗增殖活性，并抑制人类横纹肌肉瘤细胞的增殖。GR-891 可诱导人类横纹肌肉瘤细胞发生终末肌源性分化，包括调节 desmin 和 vimentin 的表达。GR-891 可被激酶磷酸化、整合到 RNA 中并释放丙烯醛。GR-891 可用于癌症及横纹肌肉瘤的研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

GR-891

GR-891 Chemical Structure

CAS No. : 138969-77-4

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

GR-891 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

GR-891 is an acyclic nucleoside 5-Fluorouracil (HY-90006) derivative. GR-891 exerts antiproliferative activity in human cancer cells and inhibits proliferation of human rhabdomyosarcoma cells. GR-891 induces terminal myogenic differentiation in human rhabdomyosarcoma cells, including modulation of desmin and vimentin expression. GR-891 is phosphorylated by kinases, incorporated into RNA, and releases acrolein. GR-891 can be used for the research of cancer and rhabdomyosarcoma[1][2].

体外研究
(In Vitro)

GR-891 (22.5-90 µM；6 天) 可呈时间和剂量依赖性抑制人横纹肌肉瘤 RD 细胞的增殖；连续处理 6 天后，各测试浓度下细胞存活率均维持在 90% 以上，且复处理后会出现不可逆的生长抑制[2]。
GR-891 (22.5-90 µM；6 天) 可诱导人横纹肌肉瘤 RD 细胞发生终末肌源性分化，其特征为成熟肌细胞的形态学、扫描电镜 (SEM) 和透射电镜 (TEM) 特征，包括形成肌管样巨细胞，在处理 6 天后该细胞占细胞总数的比例最高可达 65%[2]。
GR-891 (22.5-90 µM；6 天) 可调节人横纹肌肉瘤 RD 细胞中中间丝蛋白的表达；其中 22.5 µM 和 45 µM 的浓度可显著增加结蛋白阳性细胞比例 (分别升至 68.8% 和 63.0%)，同时显著降低波形蛋白阳性细胞比例 (分别降至 40.8% 和 57.5%)，该结果与肌源性分化一致[2]。
GR-891 (22.5-90 µM；6 天) 在浓度为 22.5、45 或 90 µmol l-1 的条件下处理人横纹肌肉瘤 RD 细胞 6 天后，不会改变 mdr1 mRNA 的表达[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

GR-891 相关抗体:

Cell Proliferation Assay[2]

Cell Line: human rhabdomyosarcoma RD cell line
Concentration: 22.5 μM; 45 μM; 90 μM
Incubation Time: 6 days
Result: Caused substantial inhibition of RD cell proliferation during the first 3 days, with inhibition sustained until day 6.
Maintained cell viability >90% for all concentrations after 6 days of treatment.
Resulted in irreversible complete growth suppression when cells were retreated for 6 days after initial 3-day treatment and recovery.

Cell Differentiation Assay[2]

Cell Line: human rhabdomyosarcoma RD cell line
Concentration: 22.5 μM; 45 μM; 90 μM
Incubation Time: 6 days
Result: Induced elongated morphology, parallel/whorl-like growth patterns, and up to 65% multinucleated myotube-like giant cells in RD cells after 3-6 days of treatment at 22.5 µM or 45 µM.
Revealed increased cytoplasm, stronger substrate adhesion, increased phylopodia/membrane folds, and parallel myofibrils via SEM at 22.5 µM or 45 µM.
Showed multinucleated giant cells with increased cytoplasmic organelles, glycogen deposits, elongated mitochondria, lipid vesicles, organized intermediate filaments, and parallel myofilament bundles with Z-band-like electron-dense zones via TEM at 22.5 µM or 45 µM.
Exhibited prominent differentiation features at 22.5 µM and 45 µM, which were also observed in retreated cultures.

RT-PCR[2]

Cell Line: human rhabdomyosarcoma RD cell line
Concentration: 22.5 μM; 45 μM; 90 μM
Incubation Time: 6 days
Result: Showed no change in the intensity of mdr1 PCR product compared to untreated cells after 6 days of treatment at any concentration.
Produced consistent β2-microglobulin PCR product intensity across all samples, confirming RNA integrity.
体内研究
(In Vivo)

GR-891 (compound 2b) (200 mg/kg；腹腔注射；每日 1 次；连续 7 天) 在健康瑞士白化小鼠中未表现出明显的体内毒性，该剂量与 5-FU 的 LD50 (200 mg/kg) 相当，且未观察到与治疗相关的发病或死亡情况[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss albino (female, adult, 26-27 g average weight, healthy)[1]
Dosage: 200 mg/kg (5-FU LD50 equivalent)
Administration: i.p.; daily; 7 days
Result: Showed no definite weight change tendency.
Showed no changes in physical appearance (no hair loss, no anal zone irritation).
Showed no reduction in vital activity.
Caused no treatment-related death; animals died only at the end of their natural biological cycle.
分子量

290.29

Formula

C12H19FN2O5
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

GR-891 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GR-891138969-77-4GR891GR 891ApoptosisvimentindesminRNAhuman rhabdomyosarcoma cellsmdr1 genefibronectinkinaseshuman cancer cellsSwiss albino micehuman rhabdomyosarcoma RD cellsInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
GR-891
目录号：
HY-182403
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z