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  3. Green DND-26 (solution)

Green DND-26 (solution) 为绿色荧光标记的溶酶体探针,具有 504/511 nm 的最大激发/发射波长。结构上由一个荧光基团和相连的弱碱基构成,可自由穿过细胞膜,一般聚集在球形细胞器上,适用于观察溶酶体内部生物合成及相关发病机理。
溶剂及浓度:DMSO:2 mM

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Green DND-26 (solution)

Green DND-26 (solution) Chemical Structure

CAS No. : 220524-71-0

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Other Forms of Green DND-26 (solution):

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Green DND-26 (solution) is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes[1].
Solvent and concentration: DMSO: 2 mM

体外研究
(In Vitro)

Green DND-26 工作液的配制
一、储备液的配制
1.1 将溶液恢复至室温,瞬时离心浓缩至管底。
1.2 稀释使用介质或适当的缓冲液 (如 PBS) 将 2 mM 存储溶液添加到工作溶液中。推荐工作液浓度为 50-100 nM。
注意:请根据实际情况调整 Green DND-26 工作液的浓度,即用即用。
细胞染色
2.1 For 悬浮细胞:4℃,1000 g 离心 3-5 分钟,弃上清。PBS 洗涤2次,每次 5 分钟。
贴壁细胞:弃去细胞培养基,加入胰蛋白酶解离细胞,制成单细胞悬液。4℃ 1000 g 离心 3-5 分钟,弃上清。PBS 洗涤 2 次,每次 5 分钟。
2.2 加入1mL Green DND-26 工作液,室温孵育 30 分钟。
2.3 4℃ 400 g 离心 3-4 分钟,弃上清。
2.4 PBS 洗涤 2 次,每次 5 分钟。
2.5 用无血清细胞培养基或 PBS 重悬细胞,荧光显微镜或流式细胞仪检测流式细胞仪。
储存
-20 ℃ 避光保存

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

362.23

Formula

C18H25BF2N4O

CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Green DND-26 (solution)
目录号:
HY-DY1082
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