2. Others Apoptosis
  3. Fluorescent Dye Caspase
  4. IETDC

IETDC 是一种 caspase 8 探针底物。IETDC 可被活化的 caspase 8 切割并释放 D-半胱氨酸。IETDC 释放的 D-半胱氨酸可与 HCBT 结合，在原位生成萤火虫荧光素，同时伴随 H2O2 介导的 6-羟基-2-氰基苯并噻唑释放产生生物发光信号。IETDC 可用于急性炎症相关研究。

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IETDC

IETDC Chemical Structure

CAS No. : 1476735-95-1

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IETDC 相关抗体

Top Publications Citing Use of Products

查看 Caspase 亚型特异性产品:

查看所有亚型
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

IETDC is a caspase 8 probe substrate. IETDC is cleaved by activated caspase 8 to release D-cysteine. The D-cysteine released by IETDC binds to HCBT to generate firefly luciferin in situ, accompanied by a bioluminescent signal produced by H2O2-mediated release of 6-hydroxy-2-cyanobenzothiazole. IETDC is applicable to studies related to acute inflammation[1].

IC50 & Target

Caspase-8

 

体外研究
(In Vitro)

IETDC (5 μM; 60 min) 可被重组半胱天冬酶 8 选择性切割，但不会被半胱天冬酶 3 或半胱天冬酶 9 切割，进而释放 D-半胱氨酸；D-半胱氨酸可与 HCBT 反应生成荧光素，使生物发光信号增强约 27 倍，且该反应可被泛半胱天冬酶抑制剂完全抑制[1]。
IETDC (10 μM; 60 min) 是一种“与”型分子逻辑门的组成部分，仅在与 PCL-2、过氧化氢和活化的半胱天冬酶 8 共同作用时，可使生物发光信号增强约 18 倍；而若抑制过氧化氢介导的 PCL-2 切割或半胱天冬酶 8 介导的 IETDC 切割，该信号会被阻断[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IETDC 相关抗体:
体内研究
(In Vivo)

IETDC (腹腔注射；单次给药；与 0.05 μmol HCBT 联合给药；0.05 μmol)，与 HCBT 联合给药后，可在脂多糖诱导的急性炎症雌性 FVB-luc+小鼠中使生物发光信号升高 18 倍，而泛半胱天冬酶抑制剂预处理可使该信号升高幅度降低 34%[1]。
IETDC (0.05 μmol; 腹腔注射; 单剂量; 与 0.05 μmol PCL-2 联合给药) 与 PCL-2 联合给药后，可在脂多糖诱导的急性炎症雌性 FVB-luc+ 小鼠中使生物发光信号升高 2.7 倍；而与抗坏血酸和泛半胱天冬酶抑制剂联合处理可使该信号减弱 30%[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: FVB-luc+ (female, 2-5 months old)[1]
Dosage: 0.05 μmol (co-administered with 0.05 μmol HCBT)
Administration: i.p.; single dose
Result: Produced an 18-fold increase in bioluminescent signal in lipopolysaccharide-treated mice compared to saline-treated controls.
Caused a 34% attenuation of the elevated bioluminescent signal when pre-treated with z-VD(OMe)-OPh.
Animal Model: FVB-luc+ (female, 2-5 months old)[1]
Dosage: 0.05 μmol (co-administered with 0.05 μmol PCL-2)
Administration: i.p.; single dose
Result: Produced a 2.7-fold increase in bioluminescent signal in lipopolysaccharide-treated mice compared to saline-treated controls.
Caused a 30% attenuation of the elevated bioluminescent signal when co-treated with ascorbic acid and z-VD(OMe)-OPh.
分子量

741.81

Formula

C32H47N5O13S
CAS 号
Sequence

Cbz-Ile-{Glu(Me)}-Thr-{Asp(Me)}-{d-Cys}
Sequence Shortening

Cbz-I-{Glu(Me)}-T-{Asp(Me)}-{d-Cys}
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

IETDC 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IETDC1476735-95-1Fluorescent DyeCaspaseH2O2female FVB-luc+ miceD-cysteinepan-caspase inhibitorcaspase 3firefly luciferincaspase 96-hydroxy-2-cyanobenzothiazolecaspase 8acute inflammationInhibitorinhibitorinhibit

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