2. Academic Validation
  3. A Genetically Encoded Allysine for the Synthesis of Proteins with Site-Specific Lysine Dimethylation

A Genetically Encoded Allysine for the Synthesis of Proteins with Site-Specific Lysine Dimethylation

  • Angew Chem Int Ed Engl. 2017 Jan 2;56(1):212-216. doi: 10.1002/anie.201609452.
Zhipeng A Wang 1,  Yu Zeng 1,  Yadagiri Kurra 1,  Xin Wang 2,  Jeffery M Tharp 1,  Erol C Vatansever 1,  Willie W Hsu 1,  Susie Dai 2,  Xinqiang Fang 3,  Wenshe R Liu 1
Affiliations

Affiliations

  • 1 Department of Chemistry, Texas A & M University, Corner of Ross and Spence Streets, College Station, TX 77843, USA.
  • 2 Department of Plant Pathology and Microbiology, Institute for Plant Genomics, Office of the Taxes State Chemist, Department of Veterinary Pathobiology, College Station, TX, 77843, USA.
  • 3 Fujian Institute of Research on the Structure of Matter, Chinese Academy of Science, Fuzhou, Fujian, 350002, P.R. China.
PMID: 27910233 DOI: 10.1002/anie.201609452
Abstract

Using the amber suppression approach, Nϵ -(4-azidobenzoxycarbonyl)-δ,ϵ-dehydrolysine, an allysine precursor is genetically encoded in E. coli. Its genetic incorporation followed by two sequential biocompatible reactions allows convenient synthesis of proteins with site-specific lysine dimethylation. Using this approach, dimethyl-histone H3 and p53 proteins have been synthesized and used to probe functions of epigenetic Enzymes including Histone Demethylase LSD1 and Histone Acetyltransferase Tip60. We confirmed that LSD1 is catalytically active toward H3K4me2 and H3K9me2 but inert toward H3K36me2, and methylation at p53 K372 directly activates TIP60 for its catalyzed acetylation at p53 K120.

Keywords

allysine; amber suppression; dimethyllysine; genetic code expansion; lysine dimethylation.

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