2. Academic Validation
  3. Supercritical fluid chromatography-tandem mass spectrometry-assisted methodology for rapid enantiomeric analysis of fenbuconazole and its chiral metabolites in fruits, vegetables, cereals, and soil

Supercritical fluid chromatography-tandem mass spectrometry-assisted methodology for rapid enantiomeric analysis of fenbuconazole and its chiral metabolites in fruits, vegetables, cereals, and soil

  • Food Chem. 2018 Feb 15:241:32-39. doi: 10.1016/j.foodchem.2017.08.038.
Yan Tao 1,  Zuntao Zheng 2,  Yang Yu 3,  Jun Xu 1,  Xingang Liu 1,  Xiaohu Wu 1,  Fengshou Dong 4,  Yongquan Zheng 5
Affiliations

Affiliations

  • 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
  • 2 Institute for the Control of Agrochemicals, Ministry of Agriculture, Beijing 100125, PR China.
  • 3 Solid Waste and Chemicals Management Center, Ministry of Environmental Protection, Beijing 100029, PR China.
  • 4 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: dongfengshou@caas.cn.
  • 5 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: zhengyongquan@ippcaas.cn.
PMID: 28958535 DOI: 10.1016/j.foodchem.2017.08.038
Abstract

Here, we developed a rapid and robust supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) method for simultaneous detection of fenbuconazole and its chiral metabolites in fruit, vegetable, cereal, and soil. Baseline separation of six stereoisomers was achieved on an amylose tris-(3,5-dimethylphenylcarbamate)-coated chiral column in 4.0min using a mobile phase composed of CO2/ethanol (flow rate of 1.8mL/min). Ionisation efficiency and sensitivity was optimized with a post-column compensation solvent (0.1% formic acid/methanol). Target analytes were extracted with acetonitrile and purified using dispersive solid phase extraction sorbents. Six stereoisomers at three concentrations (5, 50, and 250µg/kg) achieved satisfactory recoveries (76.3-104.6%) with RSDs≤11.5% Excellent linearities (R2≥0.9963) and the limits of quantification (LOQs, 0.13-3.31μg/kg) were established for all six stereoisomers. Results show that the proposed method is suitable for routine detection of six stereoisomers of fenbuconazole and its chiral metabolites in food and environmental samples.

Keywords

Chiral; Enantioseparation; Fenbuconazole; Metabolites; SFC-MS/MS.

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