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  3. Method to Study Skin Cancer: Two-Stage Chemically Induced Carcinogenesis in Mouse Skin

Method to Study Skin Cancer: Two-Stage Chemically Induced Carcinogenesis in Mouse Skin

  • Methods Mol Biol. 2020:2154:231-238. doi: 10.1007/978-1-0716-0648-3_19.
Andrei N Mardaryev 1
Affiliations

Affiliation

  • 1 Centre for Skin Sciences, School of Chemistry and Biosciences, Faculty of Life Science, University of Bradford, Bradford, UK. a.mardaryev@bradford.ac.uk.
PMID: 32314221 DOI: 10.1007/978-1-0716-0648-3_19
Abstract

Two-stage chemical carcinogenesis method is widely used to elucidate genetic and molecular changes that lead to skin Cancer development, as well as to test chemotherapeutic properties of novel drugs. This protocol allows researchers to reliably induce benign papilloma development and their conversion to squamous cell carcinoma in the skin of susceptible mouse strains in response to a single dose of carcinogen 2,4-dimethoxybenzaldehyde (DMBA) and repetitive applications of tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA).

Keywords

Chemical carcinogenesis; DMBA; Papilloma; Skin; Squamous cell carcinoma; TPA.

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