2. Academic Validation
  3. MacConkey broth purple provides an efficient MPN estimation method for Shigatoxigenic Escherichia coli

MacConkey broth purple provides an efficient MPN estimation method for Shigatoxigenic Escherichia coli

  • J Microbiol Methods. 2021 Feb:181:106132. doi: 10.1016/j.mimet.2020.106132.
Kathryn M Wright 1,  Peter J Wright 2,  Nicola J Holden 3
Affiliations

Affiliations

  • 1 Cell & Molecular Sciences, the James Hutton Institute, Dundee DD2 5DA, United Kingdom.
  • 2 Ecology and Conservation, Marine Scotland Science, 375 Victoria Road, Aberdeen AB11 9DB, United Kingdom.
  • 3 Cell & Molecular Sciences, the James Hutton Institute, Dundee DD2 5DA, United Kingdom; SRUC, Ferguson Building, Craibstone Estate, Aberdeen AB21 9YA, United Kingdom. Electronic address: nicola.holden@sruc.ac.uk.
PMID: 33370554 DOI: 10.1016/j.mimet.2020.106132
Abstract

MacConkey broth purple provides a more efficient method for Most Probable Number estimation for Shigatoxigenic Escherichia coli (E.coli) than the process of Bacterial enrichment in buffered peptone water followed by detection on MacConkey agar, since it is a single-step process that gives comparable results in plant extracts.

Keywords

Detectable limit; Food-borne pathogens; Fresh produce; Presumptive identification.

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