2. Academic Validation
  3. A Blood-Responsive AIE Bioprobe for the Ultrasensitive Detection and Assessment of Subarachnoid Hemorrhage

A Blood-Responsive AIE Bioprobe for the Ultrasensitive Detection and Assessment of Subarachnoid Hemorrhage

  • Adv Sci (Weinh). 2023 Mar;10(8):e2205435. doi: 10.1002/advs.202205435.
Maliang Tao 1 2,  Jian Mao 3,  Yun Bao 3,  Fan Liu 3,  Yiying Mai 4,  Shujuan Guan 1 2,  Shihua Luo 5,  Yifang Huang 6,  Zixiong Li 1 2,  Yuan Zhong 1 2,  Binbin Wei 1 2,  Jun Pan 3,  Qian Wang 1 2,  Lei Zheng 1 2,  Bo Situ 1 2
Affiliations

Affiliations

  • 1 Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • 2 Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • 3 Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • 4 The Second Clinical College, Southern Medical University, Guangzhou, 510515, China.
  • 5 Center for Clinical Laboratory Diagnosis and Research, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, China.
  • 6 Department of Clinical Laboratory, the First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
PMID: 36683187 DOI: 10.1002/advs.202205435
Abstract

Subarachnoid hemorrhage (SAH) is a severe subtype of stroke caused by the rupturing of blood vessels in the brain. The ability to accurately assess the degree of bleeding in an SAH model is crucial for understanding the brain-damage mechanisms and developing therapeutic strategies. However, current methods are unable to monitor microbleeding owing to their limited sensitivities. Herein, a new bleeding assessment system using a bioprobe TTVP with aggregation-induced emission (AIE) characteristics is demonstrated. TTVP is a water-soluble, small-molecule probe that specifically interacts with blood. Taking advantage of its AIE characteristics, cell membranes affinity, and albumin-targeting ability, TTVP fluoresces in bleeding areas and detects the presence of blood with a high signal-to-noise (S/N) ratio. The degree of SAH bleeding in an endovascular perforation model is clearly evaluated based on the intensity of the fluorescence observed in the brain, which enables the ultrasensitive detection of mirco-bleeding in the SAH model in a manner that outperforms the current imaging strategies. This method serves as a promising tool for the sensitive analysis of the degree of bleeding in SAHs and Other hemorrhagic diseases.

Keywords

aggregation-induced emission; biological probe; endovascular perforation model; strokes; subarachnoid hemorrhage.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z