2. NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. NF-κB Heme Oxygenase (HO) Interleukin Related Reactive Oxygen Species (ROS) NO Synthase
  4. Nrf2 activator-24

Nrf2 activator-24 是一种具有抗炎抗氧化活性的 Nrf2 激活剂。Nrf2 activator-24 可促进 Nrf2 的核转位，进而诱导下游抗氧化和细胞保护基因的表达。Nrf2 activator-24 可抑制角质形成细胞中细胞因子驱动的炎症反应。Nrf2 activator-24 可减轻巨噬细胞中的炎症、亚硝化和氧化应激反应。Nrf2 activator-24 可缓解 DNCB 诱导的小鼠模型中的局部炎症及特应性皮炎样症状。Nrf2 activator-24 可用于特应性皮炎的相关研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Nrf2 activator-24

Nrf2 activator-24 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

Nrf2 activator-24 相关抗体

Top Publications Citing Use of Products

查看 NF-κB 亚型特异性产品:

查看所有亚型
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

查看 Heme Oxygenase (HO) 亚型特异性产品:

查看所有亚型
HO-1 HO-2

查看 Interleukin Related 亚型特异性产品:

查看所有亚型
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

查看 NO Synthase 亚型特异性产品:

查看所有亚型
nNOS iNOS eNOS

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Nrf2 activator-24 is a Nrf2 activator with anti-inflammatory and antioxidant activities. Nrf2 activator-24 promotes the nuclear translocation of Nrf2, thereby inducing the expression of downstream antioxidant and cytoprotective genes. Nrf2 activator-24 inhibits cytokine-driven inflammatory responses in keratinocytes. Nrf2 activator-24 attenuates inflammation, nitrosation and oxidative stress responses in macrophages. Nrf2 activator-24 alleviates local inflammation and atopic dermatitis-like symptoms in DNCB-induced mouse models. Nrf2 activator-24 can be used in research related to atopic dermatitis[1].

体外研究
(In Vitro)

Nrf2 activator-24 (compound 5w) (6 h) 可在基因工程化 U2OS 细胞中强效诱导 Nrf2 核转位，其 EC50 为 4.9 nM[1]。
Nrf2 activator-24 (0-1 μM; 24 h) 对人角质形成细胞 HaCaT 无细胞毒性[1]。
Nrf2 activator-24 (0.003-0.1 μM; 0-24 h) 可在处理后 3 h 诱导人角质形成细胞 HaCaT 中 Nrf2 核转位达到峰值，此后直至 24 h 其水平逐渐下降[1]。
Nrf2 activator-24 (0.03-1 μM；6-12 h) 可在人角质形成细胞 HaCaT 中诱导 Nrf2、HO-1 和 GCLM 蛋白表达呈浓度依赖性升高，在 1 μM 时效应达到最大值[1]。
Nrf2 activator-24 (0.03-1 μM；6 h) 可在人角质形成细胞 HaCaT 中诱导 HMOX1、GCLM、GCLC 和 NQO1 的 mRNA 表达呈浓度依赖性升高，在 1 μM 时作用达到峰值[1]。
Nrf2 activator-24 (0.03-1 μM; 3 h) 可浓度依赖性地抑制经 TNF-α+IFN-γ 刺激的人角质形成细胞 HaCaT 中 IL-1β 的蛋白表达，IL-6 的分泌[1]。
Nrf2 activator-24 (0.03-1 μM; 6 h) 可浓度依赖性地抑制经 TNF-α+IFN-γ 刺激的人角质形成细胞 HaCaT 中 IL6、TNF、CCL17 和 CCL22 的 mRNA 表达[1]。
Nrf2 activator-24 (0.03-1 μM; 3 h) 可浓度依赖性地抑制 LPS (HY-D1056) 刺激的 Raw264.7 小鼠巨噬细胞中 iNOS 蛋白的表达[1]。
Nrf2 activator-24 (0.03-1 μM; 3 h) 可浓度依赖性地抑制 LPS 刺激的 Raw264.7 小鼠巨噬细胞中 NO 的生成[1]。
Nrf2 activator-24 (0.1 μM; 9 h) 可有效降低 Raw264.7 小鼠巨噬细胞中由 H2O2 诱导的细胞内 ROS 水平[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Nrf2 activator-24 相关抗体:

Cell Cytotoxicity Assay[1]

Cell Line: HaCaT human keratinocytes
Concentration: up to 1 μM
Incubation Time: 24 h
Result: Showed no cytotoxic effects on HaCaT keratinocytes at concentrations up to 1 μM.

Western Blot Analysis[1]

Cell Line: HaCaT human keratinocytes
Concentration: 0.003, 0.01, 0.03, 0.1 μM
Incubation Time: 3 h
Result: Increased nuclear Nrf2 in a concentration-dependent manner.
Induced a significant 3.60 ± 0.11-fold increase in nuclear Nrf2 at 0.003 μM.

Western Blot Analysis[1]

Cell Line: HaCaT human keratinocytes
Concentration: 0.03, 0.1, 0.3, 1 μM
Incubation Time: 6 h (Nrf2); 12 h (HO-1, GCLM)
Result: Increased Nrf2 protein accumulation to 5.71 ± 0.10-fold at 1 μM (6 h treatment).
Upregulated HO-1 and GCLM expression by 10.58 ± 0.74-fold and 3.44 ± 0.11-fold, respectively, at 1 μM (12 h treatment).

Western Blot Analysis[1]

Cell Line: TNF-α+IFN-γ-stimulated HaCaT human keratinocytes
Concentration: 0.03, 0.1, 0.3, 1 μM
Incubation Time: 3 h pretreatment; 24 h cytokine stimulation
Result: Suppressed TNF-α+IFN-γ-induced IL-1β protein expression in a concentration-dependent manner.

Western Blot Analysis[1]

Cell Line: LPS-stimulated Raw264.7 mouse macrophages
Concentration: 0.03, 0.1, 0.3, 1 μM
Incubation Time: 3 h pretreatment; 24 h LPS stimulation
Result: Suppressed LPS-induced iNOS protein expression in a concentration-dependent manner.
体内研究
(In Vivo)

Nrf2 activator-24 (0.1-0.5%；局部给药；每 2 天 1 次；第 8-14 天) 可有效缓解 DNCB 诱导的 BALB/c 小鼠特应性皮炎样症状[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (6-week-old male, DNCB-induced atopic dermatitis model)[1]
Dosage: 0.1%; 0.5%
Administration: topical; every other day; Days 8-14, 1 hour after DNCB challenge
Result: Significantly reduced dermatitis scores.
Significantly suppressed scratching behavior.
Reduced spleen and lymph node weights in a concentration-dependent manner.
Significantly lowered plasma IgE levels.
Significantly attenuated epidermal thickening.
Significantly reduced eosinophil infiltration in dorsal skin.
Significantly reduced mast cell infiltration in dorsal skin (0.5% group); showed near-significant reduction (0.1% group).
Significantly reduced skin IL-6 protein levels in a concentration-dependent manner.
Significantly restored skin loricrin protein levels.
分子量

360.09

Formula

C14H10Cl2O2Se
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Nrf2 activator-24 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nrf2 activator-24NF-κBHeme Oxygenase (HO)Interleukin RelatedReactive Oxygen Species (ROS)NO SynthaseNuclear factor-κBNuclear factor-kappaBHeme OxygenaseILNitric oxide synthasesNOSkeratinocytesNrf2BALB/c micemacrophagesHaCaT human keratinocytesDNCB-induced mouse modelatopic dermatitisRaw264.7 mouse macrophagesU2OS cellsantioxidant enzymesInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Nrf2 activator-24
目录号：
HY-182040
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z