2. Cytoskeleton
  3. Arp2/3 Complex
  4. Phallacidin

Phallacidin  (Synonyms: 羧基二羟鬼笔毒肽)

目录号: HY-P2031
一键复制产品信息 纯度: 92.4%
COA 产品使用指南 技术支持

Phallacidin 是一种天然的双环七肽，来源于毒蘑菇 Amanita phalloides。Phallacidin 特异性、高亲和力地结合于丝状肌动蛋白，其 Kd 为 20 nM。Phallacidin 与 F-肌动蛋白结合后，能强烈抑制其解聚，稳定微丝结构，防止其被细胞松弛素等药物破坏。Phallacidin 当与荧光基团偶联后，可作为 F-肌动蛋白的特异性荧光探针，用于在荧光显微镜下清晰显示细胞骨架中肌动蛋白的分布 (如应力纤维、皮层外周带)。

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Custom Peptide Synthesis

Phallacidin

Phallacidin Chemical Structure

CAS No. : 26645-35-2

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规格 价格 是否有货 数量
1 mg ￥8900
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Customer Review

Phallacidin 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Phallacidin is a natural bicyclic heptapeptide derived from the poisonous mushroom Amanita phalloides. Phallacidin binds to filamentous actin specifically with high affinity, with a Kd of 20 nM. After binding to F-actin, Phallacidin strongly inhibits its depolymerization, stabilizes microfilament structures, and prevents their disruption by drugs such as cytochalasins. When conjugated with a fluorophore, Phallacidin serves as a specific fluorescent probe for F-actin, which is used to clearly visualize the distribution of actin in the cytoskeleton (e.g., stress fibers, cortical peripheral bands) under fluorescence microscopy[1][2].

体外研究
(In Vitro)

phallacidin (0.3 μM; 3-24 h) 预处理可抑制培养的小牛肺动脉内皮单层细胞中 α-凝血酶诱导的肌动蛋白细胞骨架重排[1]。
用 phallacidin (0.3 μM; 3-24 h) 预处理可显著减弱 α-凝血酶诱导的培养小牛肺动脉内皮单层细胞的 125I-albumin 通透性升高，且不改变基础通透性[1]。
Phallacidin 可特异性标记 Oedogonium 属绿藻细胞有丝分裂纺锤体动粒区域内的聚合态 F-actin[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Phallacidin 相关抗体:
分子量

846.90

Formula

C37H50N8O13S
CAS 号
性状

固体

颜色

White to off-white

Sequence

Ala-Trp-Leu-Val-{D-Asp}-Cys-{Hyp} (Sulfide bridge: Trp2- Cys6)
Sequence Shortening

AWLV-{D-Asp}-C-{Hyp} (Sulfide bridge: Trp2- Cys6)
中文名称

羧基二羟鬼笔毒肽
结构分类
初始来源

Amanita exitialis basidiocarps
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
细胞实验： 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
纯度 & 产品资料

纯度: 92.4%

COA (295 KB)

产品使用指南 (1538 KB)
参考文献

Phallacidin 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phallacidin26645-35-2羧基二羟鬼笔毒肽Arp2/3 ComplexActin-related protein 2/3 complexalbuminF-actindiatom cellsthrombincalf pulmonary artery endothelial monolayersendothelial monolayersOedogonium spp. green algal cellsactin oligomersmitotic spindlesactin filamentsInhibitorinhibitorinhibit

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