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  4. Phos-FLAG

Phos-FLAG 是一种磷酸化 FLAG 肽。Phos-FLAG 可与代谢整合至细胞表面聚糖及器官糖蛋白中的叠氮基团发生施陶丁格连接反应，对细胞表面叠氮化物的存在进行定量分析。Phos-FLAG 可检测分离细胞及器官裂解物中经叠氮标记的聚糖。

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Phos-FLAG

Phos-FLAG Chemical Structure

CAS No. : 403481-00-5

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Phos-FLAG 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Phos-FLAG is a phosphorylated FLAG peptide. Phos-FLAG can undergo a Staudinger-like linkage reaction with azide groups metabolized into cell surface glycans and organ glycoproteins, enabling quantitative analysis of the presence of cell surface azides. Phos-FLAG can detect azide-labeled glycans in isolated cell and organ lysates[1].

体内研究
(In Vivo)

Phos-Flag (250-500 μM; ex vivo incubation; 1-12 h; room temperature) 可在体外介导小鼠脾细胞和器官糖蛋白上代谢引入的叠氮基团发生特异性、可检测的施陶丁格连接反应，产生具有剂量依赖性和组织特异性的标记信号[1]。
Phos-Flag (16 μmol; i.p.; single dose; 24 hours after final Ac4ManNAz injection) 可在体内成功介导与小鼠脾细胞上代谢引入叠氮基团的施陶丁格连接，产生可检测的细胞表面 Flag 表位，且未观察到毒性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Es1^e/Es1^e mice (plasma esterase-deficient, male and female); B6D2F1 mice (wild-type, male and female)[1]
Dosage: 250 μM (ex vivo incubation for 1 h); 500 μM (ex vivo incubation for 6-12 h)
Administration: ex vivo incubation; 1 h (250 μM); 6-12 h (500 μM); room temperature
Result: Exhibited a dose-dependent increase in mean fluorescence intensity (MFI) in splenocytes from Ac4ManNAz-treated mice, with MFI values significantly higher than vehicle-treated controls.
Detected discrete labelled glycoprotein bands in heart, kidney, and liver lysates from Ac4ManNAz-treated mice, but not in brain or thymus homogenates.
Significantly reduced MFI after Phos-Flag labelling in sialidase-treated splenocytes from Ac4ManNAz-treated mice, confirming azide label presence on sialic acids.
Animal Model: C57BL/6 mice[1]
Dosage: 16 μmol
Administration: i.p.; single dose; 24 hours after final Ac4ManNAz injection
Result: Showed a significant increase in MFI in splenocytes from mice treated with both Ac4ManNAz and Phos-Flag relative to untreated mice or mice treated with only one agent.
Produced a larger MFI increase in ex vivo Phos-Flag treatment of splenocytes from mice given only Ac4ManNAz compared to ex vivo treatment of splenocytes from mice given both Ac4ManNAz and in vivo Phos-Flag, indicating significant in vivo Staudinger ligation of cell-surface azides.
Observed no adverse physiological effects in mice after administration.
分子量

1359.29

Formula

C62H75N10O23P
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Phos-FLAG 相关分类

  • 摩尔计算器

  • 稀释计算器

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phos-FLAG403481-00-5Othersphosphine-functionalized Flag peptide
Inhibitorinhibitorinhibit

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