2. 重组蛋白
  3. ACLY 蛋白, Human (Sf9, His)

ACLY 蛋白, Human (Sf9, His) 是胆固醇生物合成途径中的一种重要酶。ACLY 从线粒体柠檬酸中产生乙酰辅酶 A (AcCoA)，用于胆固醇和脂肪酸的生物合成。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

ACLY Protein, Human (Sf9, His) is an important enzyme in the cholesterol biosynthetic pathway. ACLY produces acetyl-CoA (AcCoA) from mitochondrial citrate for cholesterol and fatty acid biosynthesis.
研究背景

ATP 柠檬酸裂解酶 (ACLY) 是一种重要的酶，通过从柠檬酸盐生成乙酰辅酶 A 来进行脂肪酸和胆固醇的生物合成，从而将碳水化合物与脂质代谢联系起来。ACLY 是胆固醇生物合成途径中的一种重要酶，位于 3-羟基3-甲基戊二酰辅酶 A 还原酶 (HMGCR)（他汀类药物的靶向）上游。ACLY 从线粒体柠檬酸产生乙酰辅酶 A (AcCoA)，用于胆固醇和脂肪酸的生物合成。ACLY 通过 C 末端（柠檬酸合酶同源域）形成同源四聚体，促进 ACLY 与 CoA 结合并产生 AcCoA[1]。
种属

Human
表达系统

Sf9 insect cells
标签

N-His
蛋白编号

P53396-1 (M1-M1101)
基因 ID

47
蛋白结构
N-term
His
ACLY (M1-M1101)
Accession # P53396
C-term
蛋白长度

Full Length of Isoform-1

中文名
ACLY 蛋白
同用名
ACL; Acly; ACLY_HUMAN; ATP citrate pro-S; lyase; ATP citrate lyase; ATP citrate synthase; ATP-citrate pro-S-; -lyase; ATP-citrate synthase; ATPcitrate synthase; ATPCL; Citrate cleavage enzyme; CLATP; OTTHUMP00000164773
氨基酸序列

MSAKAISEQTGKELLYKFICTTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQNLVVKPDQLIKRRGKLGLVGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYVLDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQDSVPSPRSLQGKSTTLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNT

预测分子量
123 kDa
分子量

Approximately 110 kDa, based on SDS-PAGE under reducing conditions.
纯度

≥ 85%, as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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