Wnt3a 蛋白, Mouse (His)

Wnt-3a protein is a Frizzled receptor ligand that activates TCF/LEF transcription factors in the canonical Wnt pathway, which is critical for embryonic mesoderm development, somite formation, neural tube morphogenesis, and stem cell self-renewal in vitro. Formation of a 1:1 complex with AFM ensures prolonged biological activity and may represent its physiological form. Wnt3a Protein, Mouse (His) is the recombinant mouse-derived Wnt3a, expressed by E. coli, with N-10*His labeled tag.

Wnt-3a 蛋白作为七跨膜受体卷曲家族成员的配体，可能在经典 Wnt 信号通路中发挥作用，导致 TCF/LEF 家族转录因子的激活。它对于正常胚胎中胚层发育、尾体节的形成以及发育中的神经管的形态发生至关重要。Wnt-3a 在介导体外肠隐窝基部干细胞的自我更新中发挥关键作用。此外，该蛋白质与 AFM 形成可溶性 1:1 复合物，防止寡聚化并确保延长生物活性；该复合物可能代表体液中的生理形式。作为同源寡聚物，Wnt-3a 通过二硫键连接，导致失活。它与 APCDD1、WLS、LRP6、PORCN、磷脂酰肌醇蛋白聚糖 GPC3 和 PKD1 相互作用，强调其参与多种信号传导和发育过程。

1. Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED₅₀ for this effect is 3.463 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized LRP-5, Human at 2 μg/mL (100μL/well) can bind Biotinylated Mouse Wnt3a. The ED₅₀ for this effect is ≤6.017 ng/mL.

• 
  Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED₅₀ for this effect is 3.463 ng/mL, corresponding to a specific activity is 2.888 ×10⁵ U/mg units/mg.

Mouse

E. coli

N-10*His

P27467 (S19-K352)

22416

Full Length of Mature Protein

Approximately 39 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.