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  4. Thiazolyl Blue (solution)

Thiazolyl Blue (solution)  (Synonyms: MTT (solution); Thiazolyl Blue Tetrazolium bromide (solution); Methylthiazolyldiphenyl-tetrazolium bromide (solution))

目录号: HY-DY1086
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Thiazolyl Blue (solution) (MTT (solution)) 是一种具有细胞渗透性和正电荷的四唑染料，用于检测细胞的还原性代谢。Thiazolyl Blue 被细胞通过质膜吸收，然后被细胞内的 NAD (P) H 氧化还原酶还原为甲醛。它经常用于比色测定，以测量细胞增殖，细胞毒性，和凋亡。
溶剂及浓度：PBS：5 mg/mL

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Thiazolyl Blue (solution)

Thiazolyl Blue (solution) Chemical Structure

CAS No. : 298-93-1

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1 mL ￥330
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5 mL ￥550
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Other Forms of Thiazolyl Blue (solution):

Thiazolyl Blue (solution) 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Thiazolyl Blue (solution) (MTT (solution)) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis[1].
Solvent and concentration: PBS: 5 mg/mL

体外研究
(In Vitro)

1.细胞增殖检测 (96 孔板)
1.1 接种细胞：制备的单细胞用含 10% FBS 的培养基制备悬浮液，接种于 96 孔板，每孔 1000-10000 个细胞，每孔体积 100 μL。
1.2培养细胞：37℃。5% CO2，孵育 24-72 h。
1.3 每孔加入 10 μL MTT，孵育 4 h，弃上清，悬浮细胞需先离心。
1.4 加入100 μL DMSO，震荡10分钟使晶体完全溶解
1.5 用吸光度板读数仪在 OD=562 nm 处监测吸光度的增加。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Thiazolyl Blue (solution) 相关抗体:
分子量

414.32

Formula

C18H16BrN5S
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Thiazolyl Blue (solution) 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thiazolyl Blue (solution)298-93-1MTT (solution)Thiazolyl Blue Tetrazolium bromide (solution)Methylthiazolyldiphenyl-tetrazolium bromide (solution)Fluorescent DyeInhibitorinhibitorinhibit

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