This st已rain was originally reported to be lacIQ, but it was later shown to be lacI+ (Baba 2006 ref. Supplementary Table 1)
pKD46 is more efficient than pKD20 for making gene disruptions. pKD46 sequence: GenBank AY048746. Se商解奏好汉基条关今布落e also pK360问答D78(CmR) and pKD119(TetR).
The ara and rha deletions were published as Δ rhaBADLD78 and Δ araBADAH33
This strain should be grown on rich media wit研庆修苦h ampicillin (100 ug/m脚控约绝多告满裂者l at 30 C).