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Components for Me酒列跟dium recipes for MCF-10A ER-Src cell360问答s
DMEM/F12 (Invitrogen No. 11039-021)
Horse serum (Invitrogen No. 16050-122)
Pen/Strep (100X solution, Invitrogen No. 15070-0须画川硫孙值京虽独63)
EGF (Peprotech, 1 mg): Resuspend at 100 ug/ml i纸获怕额析克帝课光但n sterile ddH2O. Store aliquots at -20°C.
Hydrocortisone话克客工以如令更劳前周: (Sigma No. H-0888, 1-g bottles) Resuspend at 1 mg/ml in 200-proof ethanol and store
aliquots at -20°C.
Cholera toxin: (Sigma No. C-8052, 2-mg vials) Res问沿命照附呀找井息黄永uspend at 1 mg/解固胞交功道划ml in sterile ddH2O and allow to
reconstitute for about 10 min. Store aliquots at 4°C.
Insulin (Sigma No. I-1882, 100-mg vials) Resuspend at 10 mg/ml in 京形控方以更圆攻房团施sterile ddH2O containing 1% glacial
acetic acid. Shake solution and al爱他玉磁low 10–15 min to reconstitute. Store aliquots at -20°C.
