A derivative spectrophotometric method for the determination of saccharin in the presence of excipients and active ingredients is described. The average correlation coefficients and relative standard deviations of the method were 0.9999 and 0.7082%, respectively, for the second-derivative and, 0.9999 and 0.5482%, respectively for the fourth-derivative methods. The average relative standard deviations for the second- and fourth-derivative determinations for saccharin oral solution were 1.37 and 0.92%, respectively, and for saccharin tablets 1.41 and 0.83%, respectively. The recoveries ranged from 96.69 to 99.34% with the second-derivative and from 95.44 to 104.76% with the fourth-derivative method for oral solution and from 95.51 to 99.72% and from 98.77 to 104.48%, respectively, for tablets.