Here, we introduce a new synthetic approach to mesoporous silica (MPS) sheets (thickness ≤ 100 nm). By using the carboxylate surfactant (N-palmitoyl-L-alanine) and Pluronic P123 as dual-templating agents, an MPS sheet was synthesised under similar conditions to SBA-15. Cytochrome c immobilised on an MPS sheet showed extremely high relative activity.