NMR characterization of a Cu(i)-bound peptide model of copper metallochaperones: Insights on the role of methionine†
Michal S. Shoshan,Deborah E. Shalev,Wencke Adriaens,Maarten Merkx,Tilman M. Hackeng,Edit Y. Tshuva
Chemical Communications Pub Date : 05/09/2011 00:00:00 , DOI:10.1039/C1CC11600B
Abstract
The first NMR structure of a Cu(I)-bound metallochaperone model with the conserved sequence MT/HCXXC revealed that at pH ∼3.0 and ∼6.8 Cu(I) binds through one Cys and the Met rather than the two Cys residues, differently than at pH ∼8.5. This suggests a possible role of Met in metal transport.
