Single atom catalysts (SACs) are a hot research area recently. Over most of the SACs, the singly dispersed atoms are the active sites, which contribute to the catalytic activities significantly compared with a catalyst with continuously packed active sites. It is essential to determine whether SACs have been successfully synthesized. Several techniques have been applied for the characterization of the dispersion states of the active sites over SACs, such as Energy Dispersive X-ray spectroscopy (EDX), Electron Energy Loss Spectroscopy (EELS), etc. In this review, the techniques for the identification of the singly dispersed sites over SACs are introduced, the advantages and limitations of each technique are pointed out, and the future research directions have been discussed. It is hoped that this review will be helpful for a more comprehensive understanding of the characterization and detection methods involved in SACs, and stimulate and promote the further development of this emerging research field.